Electron tomography reveals the endoplasmic reticulum as a membrane source for autophagosome formation

Mitsuko Hayashi-Nishino, Naonobu Fujita, Takeshi Noda, Akihito Yamaguchi, Tamotsu Yoshimori, Akitsugu Yamamoto

    Research output: Contribution to journalShort surveypeer-review

    56 Citations (Scopus)

    Abstract

    The origin and source of autophagosomal membranes are long-standing questions. By electron microscopy, we show that the endoplasmic reticulum (ER) associates with early autophagic structures called isolation membranes (IM) or phagophores in mammalian culture cells. Overexpression of a mutant of Atg4B, which causes defects in autophagosome formation, caused accumulation of ER-IM complexes. Electron tomography revealed the ER-IM complex as a subdomain of the ER forming a cradle encircling the IM, and showed that both ER and isolation membranes are interconnected.

    Original languageEnglish
    Pages (from-to)301-303
    Number of pages3
    JournalAutophagy
    Volume6
    Issue number2
    DOIs
    Publication statusPublished - 2010 Feb 16

    Keywords

    • ATG4B mutant
    • Autophagosome
    • Electron tomography
    • Endoplasmic reticulum

    ASJC Scopus subject areas

    • Molecular Biology
    • Cell Biology

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