Electron tomography reveals the endoplasmic reticulum as a membrane source for autophagosome formation

Mitsuko Hayashi-Nishino, Naonobu Fujita, Takeshi Noda, Akihito Yamaguchi, Tamotsu Yoshimori, Akitsugu Yamamoto

Research output: Contribution to journalShort surveypeer-review

60 Citations (Scopus)

Abstract

The origin and source of autophagosomal membranes are long-standing questions. By electron microscopy, we show that the endoplasmic reticulum (ER) associates with early autophagic structures called isolation membranes (IM) or phagophores in mammalian culture cells. Overexpression of a mutant of Atg4B, which causes defects in autophagosome formation, caused accumulation of ER-IM complexes. Electron tomography revealed the ER-IM complex as a subdomain of the ER forming a cradle encircling the IM, and showed that both ER and isolation membranes are interconnected.

Original languageEnglish
Pages (from-to)301-303
Number of pages3
JournalAutophagy
Volume6
Issue number2
DOIs
Publication statusPublished - 2010 Feb 16
Externally publishedYes

Keywords

  • ATG4B mutant
  • Autophagosome
  • Electron tomography
  • Endoplasmic reticulum

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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