Electron paramagnetic resonance study on nitric oxide production during brain focal ischemia and reperfusion in the rat

Shinya Sato, Teiji Tominaga, Tomoko Ohnishi, S. Tsuyoshi Ohnishi

Research output: Contribution to journalArticlepeer-review

73 Citations (Scopus)

Abstract

The production of nitric oxide (NO) during brain focal ischemia and reperfusion was using diethyldithiocarbamate (DETC)/ Fe-citrate, NO trapping reagents, and electron paramagnetic resonance spectroscopy. The NO production is potentiated after 5 min of ischemia, and is continued during 60 min of ischemia. During the reperfusion period after 60 min of ischemia, NO was also produced. However, its production during reperfusion was not observed when the ischemia time was less than 15 min. The NO signal during reperfusion after 60 min of ischemia decreased after 15 min of reperfusion. These results suggest that NO production during ischemia is a physiological reaction for increasing cerebral blood flow, while NO production during reperfusion may be related to cellular damage.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalBrain research
Volume647
Issue number1
DOIs
Publication statusPublished - 1994 May 30
Externally publishedYes

Keywords

  • Brain ischemia
  • Cerebral blood flow
  • Diethydithiocarbamate
  • Electron paramagnetic resonance
  • Nitric oxide
  • Reperfusion

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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