Electron microscopy and computational studies of Ebh, a giant cell-wall-associated protein from Staphylococcus aureus

Sou Sakamoto, Yoshikazu Tanaka, Isao Tanaka, Toshiaki Takei, Jian Yu, Makoto Kuroda, Min Yao, Toshiko Ohta, Kouhei Tsumoto

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Ebh, a giant protein found in staphylococci, contains several domains, including a large central region with 52 imperfect repeats of a domain composed of 126 amino acids. We used electron microscopy to observe the rod-like structure of a partial Ebh protein containing 10 repeating units. This is the first report of the direct observation of an Ebh structure containing a large number of repeating units, although structures containing one, two, or four repeating units have been reported. The observed structure of the partial Ebh protein was distorted and had a length of ca. 520 Å and a width of ca. 21 Å. The observed structures were consistent with those deduced from crystal structure analysis, suggesting that the Ebh domains are connected to form a rod-like structure. The crystal structure data revealed distorted, string-like features in the simulated structure of the whole-length Ebh protein. Superposition of fragments of the simulated whole-length structure of the Ebh protein onto each electron micrograph showed a high level of correlation between the observed and calculated structures. These results suggest that Ebh is composed of highly flexible filate molecules. The highly repetitive structure and the associated unique structural flexibility of Ebh support the proposed function of this protein, i.e. binding to sugars in the cell wall. This binding might result in intra-cell-wall cross-linking that contributes to the rigidity of bacterial cells.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalBiochemical and biophysical research communications
Volume376
Issue number2
DOIs
Publication statusPublished - 2008 Nov 14
Externally publishedYes

Keywords

  • Cell-wall-associated protein
  • Ebh
  • Electron microscopy
  • Flexibility
  • Staphylococcus

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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