Electrodeposition of anchored polypyrrole film on microelectrodes and stimulation of cultured cardiac myocytes

Matsuhiko Nishizawa, Hyuma Nozaki, Hirokazu Kaji, Takahiro Kitazume, Noriyuki Kobayashi, Takeshi Ishibashi, Takashi Abe

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)

Abstract

The electrically conducting polymer polypyrrole (PPy) was electrochemically deposited onto Pt microelectrodes on a polyimide (PI) substrate. Pre-modification of the PI surface with a self-assembled monolayer of octadecyltrichlorosilane-induced anisotropic lateral growth of PPy along the PI surface and enhanced adhesive strength of the PPy film. The lateral growth of PPy film around the electrode anchored the whole film to the substrate. External stimulation of cultured cardiac myocytes was carried out using the PPy-coated microelectrode. The myocytes on the microelectrode substrate were electrically conjugated to form a sheet, and showed synchronized beating upon stimulation. The threshold charge for effective stimulation of a 0.8 cm2 sheet of myocytes was around 0.2 μC, roughly corresponding to a membrane depolarization of 250 mV.

Original languageEnglish
Pages (from-to)1480-1485
Number of pages6
JournalBiomaterials
Volume28
Issue number8
DOIs
Publication statusPublished - 2007 Mar

Keywords

  • Cardiomyocyte
  • Cell culture
  • Electrical stimulation
  • Electroactive polymer
  • Electrochemistry

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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