Electroconvulsive shock increases serotonin transporter in the rat frontal cortex

Hao Wei Shen; Yohtaro Numachi; Sumiko Yoshida; Ko Fujiyama; Shigenobu Toda; Shuichi Awata; Hiroo Matsuoka; Mitsumoto Sato

doi:10.1016/S0304-3940(03)00178-2

Electroconvulsive shock increases serotonin transporter in the rat frontal cortex

Hao Wei Shen, Yohtaro Numachi, Sumiko Yoshida, Ko Fujiyama, Shigenobu Toda, Shuichi Awata, Hiroo Matsuoka, Mitsumoto Sato

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The antidepressive action of electroconvulsive shock (ECS) is thought to involve the alteration in serotonin (5-HT) neurotransmission, including the increase in 5-HT release and uptake. In our previous study, 5-HT transporter (5-HTT) mRNA expression was decreased after single and repetitive ECS in rat raphe nucleus. In the present study, we investigated the effects of single and repetitive ECS on the protein levels of 5-HTT in the frontal cortex, hippocampus and raphe nucleus of rat brain using quantitative Western blot analysis. Single ECS did not alter 5-HTT protein expression in any brain regions examined. Repetitive ECS stably increased 5-HTT protein in the frontal cortex, but not in the hippocampus and raphe nucleus. Because ECS is known to facilitate the release of neurotransmitters, our results suggest that the increased 5-HTT protein expression in the frontal cortex might be a compensatory change against the enhanced 5-HT release by ECS in presynaptic terminals.

Original language	English
Pages (from-to)	170-172
Number of pages	3
Journal	Neuroscience Letters
Volume	341
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3940(03)00178-2
Publication status	Published - 2003 May 1

Keywords

Electroconvulsive shock
Frontal cortex
Hippocampus
Raphe nucleus
Serotonin transporter
Western blotting

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0304-3940(03)00178-2

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title = "Electroconvulsive shock increases serotonin transporter in the rat frontal cortex",

abstract = "The antidepressive action of electroconvulsive shock (ECS) is thought to involve the alteration in serotonin (5-HT) neurotransmission, including the increase in 5-HT release and uptake. In our previous study, 5-HT transporter (5-HTT) mRNA expression was decreased after single and repetitive ECS in rat raphe nucleus. In the present study, we investigated the effects of single and repetitive ECS on the protein levels of 5-HTT in the frontal cortex, hippocampus and raphe nucleus of rat brain using quantitative Western blot analysis. Single ECS did not alter 5-HTT protein expression in any brain regions examined. Repetitive ECS stably increased 5-HTT protein in the frontal cortex, but not in the hippocampus and raphe nucleus. Because ECS is known to facilitate the release of neurotransmitters, our results suggest that the increased 5-HTT protein expression in the frontal cortex might be a compensatory change against the enhanced 5-HT release by ECS in presynaptic terminals.",

keywords = "Electroconvulsive shock, Frontal cortex, Hippocampus, Raphe nucleus, Serotonin transporter, Western blotting",

author = "Shen, {Hao Wei} and Yohtaro Numachi and Sumiko Yoshida and Ko Fujiyama and Shigenobu Toda and Shuichi Awata and Hiroo Matsuoka and Mitsumoto Sato",

note = "Funding Information: This study was partly supported by a Grant-in-aid for Scientific Research (B)(2) #10470200 (Y.N.) and Health Sciences Research Grants for Research on Brain Science from the Ministry of Health and Welfare #100109 (M.S.).",

year = "2003",

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T1 - Electroconvulsive shock increases serotonin transporter in the rat frontal cortex

AU - Shen, Hao Wei

AU - Numachi, Yohtaro

AU - Yoshida, Sumiko

AU - Fujiyama, Ko

AU - Toda, Shigenobu

AU - Awata, Shuichi

AU - Matsuoka, Hiroo

AU - Sato, Mitsumoto

N1 - Funding Information: This study was partly supported by a Grant-in-aid for Scientific Research (B)(2) #10470200 (Y.N.) and Health Sciences Research Grants for Research on Brain Science from the Ministry of Health and Welfare #100109 (M.S.).

PY - 2003/5/1

Y1 - 2003/5/1

N2 - The antidepressive action of electroconvulsive shock (ECS) is thought to involve the alteration in serotonin (5-HT) neurotransmission, including the increase in 5-HT release and uptake. In our previous study, 5-HT transporter (5-HTT) mRNA expression was decreased after single and repetitive ECS in rat raphe nucleus. In the present study, we investigated the effects of single and repetitive ECS on the protein levels of 5-HTT in the frontal cortex, hippocampus and raphe nucleus of rat brain using quantitative Western blot analysis. Single ECS did not alter 5-HTT protein expression in any brain regions examined. Repetitive ECS stably increased 5-HTT protein in the frontal cortex, but not in the hippocampus and raphe nucleus. Because ECS is known to facilitate the release of neurotransmitters, our results suggest that the increased 5-HTT protein expression in the frontal cortex might be a compensatory change against the enhanced 5-HT release by ECS in presynaptic terminals.

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