Electrochemicolor Imaging Using an LSI-Based Device for Multiplexed Cell Assays

Yusuke Kanno, Kosuke Ino, Hiroya Abe, Chika Sakamoto, Takehiro Onodera, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Multiplexed bioimaging systems have triggered the development of effective assays, contributing new biological information. Although electrochemical imaging is beneficial for quantitative analysis in real time, monitoring multiple cell functions is difficult. We have developed a novel electrochemical imaging system, herein, using a large-scale integration (LSI)-based amperometric device for detecting multiple biomolecules simultaneously. This system is designated as an electrochemicolor imaging system in which the current signals from two different types of biomolecules are depicted as a multicolor electrochemical image. The mode-selectable function of the 400-electrode device enables the imaging system and two different potentials can be independently applied to the selected electrodes. The imaging system is successfully applied for detecting multiple cell functions of the embryonic stem (ES) cell and the rat pheochromocytoma (PC12) cell aggregates. To the best of our knowledge, this is the first time that a real-time electrochemical mapping technique for multiple electroactive species, simultaneously, has been reported. The imaging system is a promising bioanalytical method for exploring complex biological phenomena.

Original languageEnglish
Pages (from-to)12778-12786
Number of pages9
JournalAnalytical Chemistry
Volume89
Issue number23
DOIs
Publication statusPublished - 2017 Dec 5

ASJC Scopus subject areas

  • Analytical Chemistry

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