Electrochemical/photochemical formation of enzyme patterns on glass substrates using a scanning electrochemical/confocal microscope

Daisuke Oyamatsu, Norihiro Kanaya, Hiroshi Shiku, Matsuhiko Nishizawa, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

A hybrid system consisting of a scanning electrochemical microscope (SECM) and scanning confocal microscope (SCFM) was fabricated and used for micropatterning and imaging of diaphorase immobilized on a glass substrate. Simultaneous imaging of the diaphorase spots demonstrated that the SECM can provide information on a localized enzyme reaction, while the SCFM affords information on the location of the active enzyme. By using this SECM/SCFM system, spatially selective deactivation of diaphorase was performed by inducing a local electrochemical reaction or by irradiating with the focused laser. The resulting patterns of diaphorase were simultaneously imaged with the SECM/SCFM system.

Original languageEnglish
Pages (from-to)199-204
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume91
Issue number1-3
DOIs
Publication statusPublished - 2003 Jun 1

Keywords

  • Diaphorase
  • Patterning
  • SCFM
  • SECM

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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