Electrochemical mutagen screening using microbial chip

Nobuto Matsui, Takatoshi Kaya, Kuniaki Nagamine, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)

Abstract

Electrochemical microbial chip for mutagen screening were microfabricated and characterized by scanning electrochemical microscopy (SECM). Salmonella typhimurium TA1535 with a plasmid pSK1002 carrying a umuC'-'lacZ fusion gene was used for the whole cell mutagen sensor. The TA1535/pSK1002 cells were exposed to mutagen solutions containing 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamido (AF-2), mitomycin C (MMC) or 2-aminoanthracene (2-AA) and embedded in a microcavity (5 nl) on a glass substrate using collagen gel. The β-galactosidase expression on the microbial chip was electrochemically monitored using p-aminophenyl-β-d-galactopyranoside (PAPG) as the enzymatic substrate. This system has several advantages compared with the conventional umu test: drastic reduction of the sample volume, less time-consuming for β-galactosidase detection (free from substrate reaction time) and lower detection limit for the three mutagens (AF-2, MMC, 2-AA). Finally, a multi-sample assay was carried out using the microbial array chip with four microcavities.

Original languageEnglish
Pages (from-to)1202-1209
Number of pages8
JournalBiosensors and Bioelectronics
Volume21
Issue number7
DOIs
Publication statusPublished - 2006 Jan 15

Keywords

  • Collagen
  • Electrochemical detection
  • Microbial chip
  • Mutagen
  • Scanning electrochemical microscopy
  • β-Galactosidase

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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