TY - JOUR
T1 - Electrochemical impedance probing of transcriptional TATA binding protein based on TATA box site-specific binding
AU - Chang, Haixin
AU - Li, Jinghong
N1 - Funding Information:
This work was financially supported by the National Natural Science Foundation of China ( No. 20675044 ), National Basic Research Program of China ( Nos. 2007CB310500 and 28-AZC0901 ) and China Postdoctoral Science Foundation ( No. 20080430349 ).
PY - 2009/11
Y1 - 2009/11
N2 - Electrochemical impedance probing of TATA binding protein (TBP) based on TATA box site-specific binding was described in this work. A sensitive detection of TBP was developed from TATA box DNA self assembly on the electrode and the impedance changes induced by TBP binding. Electrochemical impedance spectroscopy (EIS) probing of TBP had a sensitivity of 0.8 nM with excellent selectivity. Moreover, the interferences of triplex forming oligonucleotides (TFOs) and anticancer drug daunomycin on TBP binding to TATA box DNA were investigated by EIS. TFOs reduced the stability of TBP binding to TATA box, but daunomycin completely inhibited the TBP binding.
AB - Electrochemical impedance probing of TATA binding protein (TBP) based on TATA box site-specific binding was described in this work. A sensitive detection of TBP was developed from TATA box DNA self assembly on the electrode and the impedance changes induced by TBP binding. Electrochemical impedance spectroscopy (EIS) probing of TBP had a sensitivity of 0.8 nM with excellent selectivity. Moreover, the interferences of triplex forming oligonucleotides (TFOs) and anticancer drug daunomycin on TBP binding to TATA box DNA were investigated by EIS. TFOs reduced the stability of TBP binding to TATA box, but daunomycin completely inhibited the TBP binding.
KW - DNA
KW - Electrochemical impedance spectroscopy
KW - TATA binding protein
KW - Triplex formation oligonucleotides
UR - http://www.scopus.com/inward/record.url?scp=70350568237&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70350568237&partnerID=8YFLogxK
U2 - 10.1016/j.elecom.2009.09.004
DO - 10.1016/j.elecom.2009.09.004
M3 - Article
AN - SCOPUS:70350568237
SN - 1388-2481
VL - 11
SP - 2101
EP - 2104
JO - Electrochemistry Communications
JF - Electrochemistry Communications
IS - 11
ER -