Electrochemical impedance probing of transcriptional TATA binding protein based on TATA box site-specific binding

Haixin Chang, Jinghong Li

    Research output: Contribution to journalArticlepeer-review

    14 Citations (Scopus)

    Abstract

    Electrochemical impedance probing of TATA binding protein (TBP) based on TATA box site-specific binding was described in this work. A sensitive detection of TBP was developed from TATA box DNA self assembly on the electrode and the impedance changes induced by TBP binding. Electrochemical impedance spectroscopy (EIS) probing of TBP had a sensitivity of 0.8 nM with excellent selectivity. Moreover, the interferences of triplex forming oligonucleotides (TFOs) and anticancer drug daunomycin on TBP binding to TATA box DNA were investigated by EIS. TFOs reduced the stability of TBP binding to TATA box, but daunomycin completely inhibited the TBP binding.

    Original languageEnglish
    Pages (from-to)2101-2104
    Number of pages4
    JournalElectrochemistry Communications
    Volume11
    Issue number11
    DOIs
    Publication statusPublished - 2009 Nov 1

    Keywords

    • DNA
    • Electrochemical impedance spectroscopy
    • TATA binding protein
    • Triplex formation oligonucleotides

    ASJC Scopus subject areas

    • Electrochemistry

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