Electrochemical impedance probing of transcriptional TATA binding protein based on TATA box site-specific binding

Haixin Chang; Jinghong Li

doi:10.1016/j.elecom.2009.09.004

Electrochemical impedance probing of transcriptional TATA binding protein based on TATA box site-specific binding

Haixin Chang, Jinghong Li

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Electrochemical impedance probing of TATA binding protein (TBP) based on TATA box site-specific binding was described in this work. A sensitive detection of TBP was developed from TATA box DNA self assembly on the electrode and the impedance changes induced by TBP binding. Electrochemical impedance spectroscopy (EIS) probing of TBP had a sensitivity of 0.8 nM with excellent selectivity. Moreover, the interferences of triplex forming oligonucleotides (TFOs) and anticancer drug daunomycin on TBP binding to TATA box DNA were investigated by EIS. TFOs reduced the stability of TBP binding to TATA box, but daunomycin completely inhibited the TBP binding.

Original language	English
Pages (from-to)	2101-2104
Number of pages	4
Journal	Electrochemistry Communications
Volume	11
Issue number	11
DOIs	https://doi.org/10.1016/j.elecom.2009.09.004
Publication status	Published - 2009 Nov 1

Keywords

DNA
Electrochemical impedance spectroscopy
TATA binding protein
Triplex formation oligonucleotides

ASJC Scopus subject areas

Electrochemistry

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10.1016/j.elecom.2009.09.004

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title = "Electrochemical impedance probing of transcriptional TATA binding protein based on TATA box site-specific binding",

abstract = "Electrochemical impedance probing of TATA binding protein (TBP) based on TATA box site-specific binding was described in this work. A sensitive detection of TBP was developed from TATA box DNA self assembly on the electrode and the impedance changes induced by TBP binding. Electrochemical impedance spectroscopy (EIS) probing of TBP had a sensitivity of 0.8 nM with excellent selectivity. Moreover, the interferences of triplex forming oligonucleotides (TFOs) and anticancer drug daunomycin on TBP binding to TATA box DNA were investigated by EIS. TFOs reduced the stability of TBP binding to TATA box, but daunomycin completely inhibited the TBP binding.",

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AU - Chang, Haixin

AU - Li, Jinghong

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N2 - Electrochemical impedance probing of TATA binding protein (TBP) based on TATA box site-specific binding was described in this work. A sensitive detection of TBP was developed from TATA box DNA self assembly on the electrode and the impedance changes induced by TBP binding. Electrochemical impedance spectroscopy (EIS) probing of TBP had a sensitivity of 0.8 nM with excellent selectivity. Moreover, the interferences of triplex forming oligonucleotides (TFOs) and anticancer drug daunomycin on TBP binding to TATA box DNA were investigated by EIS. TFOs reduced the stability of TBP binding to TATA box, but daunomycin completely inhibited the TBP binding.

AB - Electrochemical impedance probing of TATA binding protein (TBP) based on TATA box site-specific binding was described in this work. A sensitive detection of TBP was developed from TATA box DNA self assembly on the electrode and the impedance changes induced by TBP binding. Electrochemical impedance spectroscopy (EIS) probing of TBP had a sensitivity of 0.8 nM with excellent selectivity. Moreover, the interferences of triplex forming oligonucleotides (TFOs) and anticancer drug daunomycin on TBP binding to TATA box DNA were investigated by EIS. TFOs reduced the stability of TBP binding to TATA box, but daunomycin completely inhibited the TBP binding.

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KW - Electrochemical impedance spectroscopy

KW - TATA binding protein

KW - Triplex formation oligonucleotides

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