Electrochemical gene-function analysis for single cells with addressable microelectrode/microwell arrays

Zhenyu Lin; Yasufumi Takahashi; Tatsuya Murata; Michiaki Takeda; Kosuke Ino; Hitoshi Shiku; Tomokazu Matsue

doi:10.1002/anie.200805743

Electrochemical gene-function analysis for single cells with addressable microelectrode/microwell arrays

Zhenyu Lin, Yasufumi Takahashi, Tatsuya Murata, Michiaki Takeda, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

(Graph Presented) To each his own: An addressable electrochemical device consisting of orthogonally arranged rows and columns of electrodes has been constructed to monitor protein expression in genetically engineered cells at the single-cell level. The response based on redox cycling reflected the different expression levels of the enzyme from individual HeLa cells transfected with a plasmid vector including secreted alkaline phosphatase.

Original language	English
Pages (from-to)	2044-2046
Number of pages	3
Journal	Angewandte Chemie - International Edition
Volume	48
Issue number	11
DOIs	https://doi.org/10.1002/anie.200805743
Publication status	Published - 2009 Mar 2

Keywords

Biosensors
Electrochemistry
High-throughput screening
Microelectrodes
Single-cell analysis

ASJC Scopus subject areas

Catalysis
Chemistry(all)

Access to Document

10.1002/anie.200805743

Cite this

Electrochemical gene-function analysis for single cells with addressable microelectrode/microwell arrays. / Lin, Zhenyu; Takahashi, Yasufumi; Murata, Tatsuya; Takeda, Michiaki; Ino, Kosuke ; Shiku, Hitoshi ; Matsue, Tomokazu.

In: Angewandte Chemie - International Edition, Vol. 48, No. 11, 02.03.2009, p. 2044-2046.

Research output: Contribution to journal › Article › peer-review

@article{09ab0eab2b0a4c51843facbdb7df13f4,

title = "Electrochemical gene-function analysis for single cells with addressable microelectrode/microwell arrays",

abstract = "(Graph Presented) To each his own: An addressable electrochemical device consisting of orthogonally arranged rows and columns of electrodes has been constructed to monitor protein expression in genetically engineered cells at the single-cell level. The response based on redox cycling reflected the different expression levels of the enzyme from individual HeLa cells transfected with a plasmid vector including secreted alkaline phosphatase.",

keywords = "Biosensors, Electrochemistry, High-throughput screening, Microelectrodes, Single-cell analysis",

author = "Zhenyu Lin and Yasufumi Takahashi and Tatsuya Murata and Michiaki Takeda and Kosuke Ino and Hitoshi Shiku and Tomokazu Matsue",

year = "2009",

month = mar,

day = "2",

doi = "10.1002/anie.200805743",

language = "English",

volume = "48",

pages = "2044--2046",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley and Sons Ltd",

number = "11",

}

TY - JOUR

T1 - Electrochemical gene-function analysis for single cells with addressable microelectrode/microwell arrays

AU - Lin, Zhenyu

AU - Takahashi, Yasufumi

AU - Murata, Tatsuya

AU - Takeda, Michiaki

AU - Ino, Kosuke

AU - Shiku, Hitoshi

AU - Matsue, Tomokazu

PY - 2009/3/2

Y1 - 2009/3/2

N2 - (Graph Presented) To each his own: An addressable electrochemical device consisting of orthogonally arranged rows and columns of electrodes has been constructed to monitor protein expression in genetically engineered cells at the single-cell level. The response based on redox cycling reflected the different expression levels of the enzyme from individual HeLa cells transfected with a plasmid vector including secreted alkaline phosphatase.

AB - (Graph Presented) To each his own: An addressable electrochemical device consisting of orthogonally arranged rows and columns of electrodes has been constructed to monitor protein expression in genetically engineered cells at the single-cell level. The response based on redox cycling reflected the different expression levels of the enzyme from individual HeLa cells transfected with a plasmid vector including secreted alkaline phosphatase.

KW - Biosensors

KW - Electrochemistry

KW - High-throughput screening

KW - Microelectrodes

KW - Single-cell analysis

UR - http://www.scopus.com/inward/record.url?scp=61849172878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61849172878&partnerID=8YFLogxK

U2 - 10.1002/anie.200805743

DO - 10.1002/anie.200805743

M3 - Article

C2 - 19191275

AN - SCOPUS:61849172878

VL - 48

SP - 2044

EP - 2046

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

SN - 1433-7851

IS - 11

ER -

Electrochemical gene-function analysis for single cells with addressable microelectrode/microwell arrays

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this