Electrochemical evaluation of sarcomeric α-actinin in embryoid bodies after gene silencing using an LSI-based amperometric sensor array

Mustafa Şen, Kosuke Ino, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

In the present study, gene analysis using siRNAs for cell differentiation of embryonic stem (ES) cells was performed using an LSI-based amperometric sensor array (Bio-LSI). CITED2 and WNT11 were silenced using siRNA, and then the effect of these genes on the differentiation of ES cells into cardiomyocytes was evaluated. For the evaluation, endogenous ALP activity and sarcomeric α-actinin were electrochemically detected using the Bio-LSI system. These results show the crucial role of these genes in determining the fate of differentiation of ES cells. These results also show that the Bio-LSI is a great tool for cell analysis. This journal is

Original languageEnglish
Pages (from-to)6337-6342
Number of pages6
JournalAnalytical Methods
Volume6
Issue number16
DOIs
Publication statusPublished - 2014 Aug 21

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

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