Electrochemical enzymatic biosensor with long-term stability using hybrid mesoporous membrane

Tetsuji Itoh, Takeshi Shimomura, Akari Hayashi, Akira Yamaguchi, Norio Teramae, Masatoshi Ono, Tatsuo Tsunoda, Fujio Mizukami, Galen D. Stucky, Taka Aki Hanaoka

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

An acetylcholinesterase-immobilized sensor unit was successfully prepared by encapsulating the enzyme within hybrid mesoporous silica membranes (F127-MST). Through a novel combination with tetracyanoquinodimethane, both acetylcholine and organophosphorus pesticides were successfully detected with high sensitivity. Furthermore, we manufactured the working prototype of an enzyme sensor with this sensor unit for detecting dichlorvos, aldicarb and parathion. At present, the detection limit in this working prototype either equaled or surpassed that of others. Also, we have the advantage of increased stability of the enzyme against the outer environment by encapsulation of the enzymes into a silica nanospace. Consequently, acetylcholinesterase immobilized in F127-MST is a practical sensor with high sensitivity, reusability, and storage stability. This journal is

Original languageEnglish
Pages (from-to)4654-4660
Number of pages7
JournalAnalyst
Volume139
Issue number18
DOIs
Publication statusPublished - 2014 Sep 21

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry
  • Environmental Chemistry

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    Itoh, T., Shimomura, T., Hayashi, A., Yamaguchi, A., Teramae, N., Ono, M., Tsunoda, T., Mizukami, F., Stucky, G. D., & Hanaoka, T. A. (2014). Electrochemical enzymatic biosensor with long-term stability using hybrid mesoporous membrane. Analyst, 139(18), 4654-4660. https://doi.org/10.1039/c4an00975d