Electrochemical DNA sensors based on polymeric indicator: Use of a poly(4-vinylpyridine) derivative bearing [Os(5,6-5,6-dimethyl-1,10- phenanthroline)2Cl]2+

Aihua Liu; Jun Ichi Anzai

Electrochemical DNA sensors based on polymeric indicator: Use of a poly(4-vinylpyridine) derivative bearing [Os(5,6-5,6-dimethyl-1,10- phenanthroline)₂Cl]²⁺

Aihua Liu, Jun Ichi Anzai

PHA - Molecular Pharmaceutical Science

Research output: Contribution to conference › Paper

Abstract

A poly(4-vinylpyridine) (PVP) derivative bearing redox-active osmium complexes, PVP-[Os(5,6-dmphen)₂Cl]²⁺ (5,6-dmphen = 5,6-dimethyl-1,10-phenanthroline), was employed as a hybridization indicator for electrochemical DNA sensors. PVP-[Os(5,6-dmphen)₂Cl]²⁺ exhibited ∼1000 times higher sensitivity than the corresponding monomeric analogue, [Os(5,6-dmphen)₃]²⁺, in DNA determination due to polymeric effects. The detection limit of the present sensor was 0.1 pM. Another merit of the polymeric indicator is that the redox potential was found to be +360 mV (vs Ag/AgCl), which is significantly lower than that reported for the monomeric analogue (+672 mV). The polymeric indicator was applicable to the discrimination of single- and double-base-mismatched DNAs from fully matched target DNA. The polymeric indicator can be removed from the electrode surface by rinsing the electrode in a high-temperature buffer for 6 min, and thus, the polymeric indicator-based DNA sensor, can.

Original language	English
Pages	423-430
Number of pages	8
Publication status	Published - 2004 Dec 1
Event	Chemical Sensors VI: Chemical and Biological Sensors and Analytical Methods - Proceedings of the International Symposium - Honolulu, HI, United States Duration: 2004 Oct 3 → 2004 Oct 8

Other

Other	Chemical Sensors VI: Chemical and Biological Sensors and Analytical Methods - Proceedings of the International Symposium
Country	United States
City	Honolulu, HI
Period	04/10/3 → 04/10/8

ASJC Scopus subject areas

Engineering(all)

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Liu, A., & Anzai, J. I. (2004). Electrochemical DNA sensors based on polymeric indicator: Use of a poly(4-vinylpyridine) derivative bearing [Os(5,6-5,6-dimethyl-1,10- phenanthroline)₂Cl]²⁺. 423-430. Paper presented at Chemical Sensors VI: Chemical and Biological Sensors and Analytical Methods - Proceedings of the International Symposium, Honolulu, HI, United States.

Electrochemical DNA sensors based on polymeric indicator : Use of a poly(4-vinylpyridine) derivative bearing [Os(5,6-5,6-dimethyl-1,10- phenanthroline)₂Cl]²⁺. / Liu, Aihua; Anzai, Jun Ichi.

2004. 423-430 Paper presented at Chemical Sensors VI: Chemical and Biological Sensors and Analytical Methods - Proceedings of the International Symposium, Honolulu, HI, United States.

Research output: Contribution to conference › Paper

Liu, A & Anzai, JI 2004, 'Electrochemical DNA sensors based on polymeric indicator: Use of a poly(4-vinylpyridine) derivative bearing [Os(5,6-5,6-dimethyl-1,10- phenanthroline)₂Cl]²⁺', Paper presented at Chemical Sensors VI: Chemical and Biological Sensors and Analytical Methods - Proceedings of the International Symposium, Honolulu, HI, United States, 04/10/3 - 04/10/8 pp. 423-430.

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abstract = "A poly(4-vinylpyridine) (PVP) derivative bearing redox-active osmium complexes, PVP-[Os(5,6-dmphen)2Cl]2+ (5,6-dmphen = 5,6-dimethyl-1,10-phenanthroline), was employed as a hybridization indicator for electrochemical DNA sensors. PVP-[Os(5,6-dmphen)2Cl]2+ exhibited ∼1000 times higher sensitivity than the corresponding monomeric analogue, [Os(5,6-dmphen)3]2+, in DNA determination due to polymeric effects. The detection limit of the present sensor was 0.1 pM. Another merit of the polymeric indicator is that the redox potential was found to be +360 mV (vs Ag/AgCl), which is significantly lower than that reported for the monomeric analogue (+672 mV). The polymeric indicator was applicable to the discrimination of single- and double-base-mismatched DNAs from fully matched target DNA. The polymeric indicator can be removed from the electrode surface by rinsing the electrode in a high-temperature buffer for 6 min, and thus, the polymeric indicator-based DNA sensor, can.",

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N2 - A poly(4-vinylpyridine) (PVP) derivative bearing redox-active osmium complexes, PVP-[Os(5,6-dmphen)2Cl]2+ (5,6-dmphen = 5,6-dimethyl-1,10-phenanthroline), was employed as a hybridization indicator for electrochemical DNA sensors. PVP-[Os(5,6-dmphen)2Cl]2+ exhibited ∼1000 times higher sensitivity than the corresponding monomeric analogue, [Os(5,6-dmphen)3]2+, in DNA determination due to polymeric effects. The detection limit of the present sensor was 0.1 pM. Another merit of the polymeric indicator is that the redox potential was found to be +360 mV (vs Ag/AgCl), which is significantly lower than that reported for the monomeric analogue (+672 mV). The polymeric indicator was applicable to the discrimination of single- and double-base-mismatched DNAs from fully matched target DNA. The polymeric indicator can be removed from the electrode surface by rinsing the electrode in a high-temperature buffer for 6 min, and thus, the polymeric indicator-based DNA sensor, can.

AB - A poly(4-vinylpyridine) (PVP) derivative bearing redox-active osmium complexes, PVP-[Os(5,6-dmphen)2Cl]2+ (5,6-dmphen = 5,6-dimethyl-1,10-phenanthroline), was employed as a hybridization indicator for electrochemical DNA sensors. PVP-[Os(5,6-dmphen)2Cl]2+ exhibited ∼1000 times higher sensitivity than the corresponding monomeric analogue, [Os(5,6-dmphen)3]2+, in DNA determination due to polymeric effects. The detection limit of the present sensor was 0.1 pM. Another merit of the polymeric indicator is that the redox potential was found to be +360 mV (vs Ag/AgCl), which is significantly lower than that reported for the monomeric analogue (+672 mV). The polymeric indicator was applicable to the discrimination of single- and double-base-mismatched DNAs from fully matched target DNA. The polymeric indicator can be removed from the electrode surface by rinsing the electrode in a high-temperature buffer for 6 min, and thus, the polymeric indicator-based DNA sensor, can.

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