Electrochemical Detection of Triglycerides Based on an Enzymatic Reaction and Electrocatalytic Oxidation with Nortropine-N-oxyl

Tetsuya Ono, Katsuhiko Sato, Yusuke Sasano, Kentaro Yoshida, Takenori Dairaku, Yoshiharu Iwabuchi, Yoshitomo Kashiwagi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

This work investigated a simple triglycerides (TGs) detection method combining an enzymatic reaction, using only lipase, and the electrocatalytic oxidation of glycerol with nortropine-N-oxyl (NNO). Tributyrin was employed as a model TG. In this method, TGs are degraded by lipase to glycerol and fatty acids, after which the glycerol reacts with NNO. Preliminary cyclic voltammetry trials demonstrated that the anodic peak current increased along with the glycerol concentration, giving currents of 53.2 and 97.3 μA (at +0.6 V vs. Ag/AgCl) in response to 10 and 100 mM glycerol, respectively. Amperometry confirmed the same response during constant potential electrolysis at+0.6 V vs. Ag/AgCl. This behavior was also observed in a system incorporating tributyrin and lipase, with increases in current proportional to the tributyrin concentration over the range of 0.1 to 10 mM.

Original languageEnglish
Pages (from-to)603-606
Number of pages4
JournalElectroanalysis
Volume31
Issue number4
DOIs
Publication statusPublished - 2019 Apr

Keywords

  • Electrocatalytic oxidation
  • Mediator
  • Nitroxyl radical
  • Sensor
  • Triglyceride

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Fingerprint Dive into the research topics of 'Electrochemical Detection of Triglycerides Based on an Enzymatic Reaction and Electrocatalytic Oxidation with Nortropine-N-oxyl'. Together they form a unique fingerprint.

Cite this