Electrochemical detection of secreted alkaline phosphatase (SEAP) from transformed hela cells using a lab-on-a-chip device based on target concentration and local redox-cycling

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports a lab-on-a-chip device having 256 individually addressable multi-detection points for high throughput single cell analysis. The chip is based on local redox-cycling and basically comprises 4 layers one on another. In order to fabricate individually addressable sensor points, two sets of microelectrode bands (row and column) were placed orthogonally to make 256 cross points where the sensor points are located. The chip device was then used to detect secreted proteins from single cells. First, the bottom surface of each detection point was modified with antibodies for the protein of interest and then single cells were captured in microwells at sensor points. The cells were incubated 4 h to secrete enough proteins to be immobilized. After which the cells were removed and the signal from the immobilized proteins were detected and amplified using redox cycling by setting the potential of electrodes to appropriate values. The results indicated that the secreted proteins were successfully detected using the chip device, which could be used for reporter gene assay and detection of some naturally secreted proteins.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1918-1920
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/TerritoryJapan
CityOkinawa
Period12/10/2812/11/1

Keywords

  • Biosensing
  • Electrochemistry
  • Lab on a chip
  • Redox cycling
  • Secreted alkaline phosphatase
  • Single cell

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Fingerprint

Dive into the research topics of 'Electrochemical detection of secreted alkaline phosphatase (SEAP) from transformed hela cells using a lab-on-a-chip device based on target concentration and local redox-cycling'. Together they form a unique fingerprint.

Cite this