Abstract
This paper reports a lab-on-a-chip device having 256 individually addressable multi-detection points for high throughput single cell analysis. The chip is based on local redox-cycling and basically comprises 4 layers one on another. In order to fabricate individually addressable sensor points, two sets of microelectrode bands (row and column) were placed orthogonally to make 256 cross points where the sensor points are located. The chip device was then used to detect secreted proteins from single cells. First, the bottom surface of each detection point was modified with antibodies for the protein of interest and then single cells were captured in microwells at sensor points. The cells were incubated 4 h to secrete enough proteins to be immobilized. After which the cells were removed and the signal from the immobilized proteins were detected and amplified using redox cycling by setting the potential of electrodes to appropriate values. The results indicated that the secreted proteins were successfully detected using the chip device, which could be used for reporter gene assay and detection of some naturally secreted proteins.
Original language | English |
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Title of host publication | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
Publisher | Chemical and Biological Microsystems Society |
Pages | 1918-1920 |
Number of pages | 3 |
ISBN (Print) | 9780979806452 |
Publication status | Published - 2012 Jan 1 |
Event | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: 2012 Oct 28 → 2012 Nov 1 |
Other
Other | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
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Country/Territory | Japan |
City | Okinawa |
Period | 12/10/28 → 12/11/1 |
Keywords
- Biosensing
- Electrochemistry
- Lab on a chip
- Redox cycling
- Secreted alkaline phosphatase
- Single cell
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering