Electrochemical Detection of Kallikrein Using a p-Methoxyaniline-conjugated Tripeptide towards Simple Diagnosis of Primary Aldosteronism

Kousuke Ohyama, Sun Sixiang, Kumi Y. Inoue, Tomokazu Matsue, Takayuki Doi

Research output: Contribution to journalArticlepeer-review

Abstract

Kallikrein is a serine protease which is involved in blood pressure regulation and is overexpressed in primary aldosteronism. We hypothesized that quick quantification of this enzyme could represent a valuable diagnostic technique. Towards this aim, we demonstrate the use of an electrochemical approach to detect and quantify kallikrein for the first time. We designed the tripeptide Pro-Phe-Arg-pMA as a substrate for kallikrein and analyzed the enzyme concentration by measuring the release of the product, p-methoxyaniline (pMA), from kallikrein-mediated hydrolysis. Released pMA was detected by electrochemical measurement with a detection limit of 0.01 units/mL. The fit of the analysis of released pMA by chronoamperometry with MichaelisMenten curves indicates that this technique offers quantitative detection.

Original languageEnglish
Pages (from-to)57-59
Number of pages3
JournalChemistry Letters
Volume49
Issue number1
DOIs
Publication statusPublished - 2020

Keywords

  • Electrochemical detection
  • Enzymatic reaction
  • Kallikrein

ASJC Scopus subject areas

  • Chemistry(all)

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