Abstract
Avidin was electrochemically deposited on a platinum electrode without loss of binding activity to biotin, and biotin-labeled glucose oxidase was immobilized on the electrode through avidin-biotin binding. The electrodes thus prepared displayed excellent properties as a glucose sensor. The oxidation current is proportional to glucose concentration from 10-4 to 10-2 M, and the sensor could be used for more than 3 months.
Original language | English |
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Pages (from-to) | 321-327 |
Number of pages | 7 |
Journal | Analytica Chimica Acta |
Volume | 289 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1994 May 10 |
Keywords
- Avidin
- Biotin
- Electrodeposition
- Enzymatic methods
- Glucose oxidase
- Immobilization
- Sensors
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy