Electrochemical deposition of avidin on the surface of a platinum electrode for enzyme sensor applications

Tomonori Hoshi, Junichi Anzai, Tetsuo Osa

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Avidin was electrochemically deposited on a platinum electrode without loss of binding activity to biotin, and biotin-labeled glucose oxidase was immobilized on the electrode through avidin-biotin binding. The electrodes thus prepared displayed excellent properties as a glucose sensor. The oxidation current is proportional to glucose concentration from 10-4 to 10-2 M, and the sensor could be used for more than 3 months.

Original languageEnglish
Pages (from-to)321-327
Number of pages7
JournalAnalytica Chimica Acta
Volume289
Issue number3
DOIs
Publication statusPublished - 1994 May 10

Keywords

  • Avidin
  • Biotin
  • Electrodeposition
  • Enzymatic methods
  • Glucose oxidase
  • Immobilization
  • Sensors

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Fingerprint

Dive into the research topics of 'Electrochemical deposition of avidin on the surface of a platinum electrode for enzyme sensor applications'. Together they form a unique fingerprint.

Cite this