Electrochemical deposition of avidin on the surface of a platinum electrode for enzyme sensor applications

Tomonori Hoshi; Junichi Anzai; Tetsuo Osa

doi:10.1016/0003-2670(94)90008-X

Electrochemical deposition of avidin on the surface of a platinum electrode for enzyme sensor applications

Tomonori Hoshi, Junichi Anzai, Tetsuo Osa

Clinical Research, Innovation and Education Center

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Avidin was electrochemically deposited on a platinum electrode without loss of binding activity to biotin, and biotin-labeled glucose oxidase was immobilized on the electrode through avidin-biotin binding. The electrodes thus prepared displayed excellent properties as a glucose sensor. The oxidation current is proportional to glucose concentration from 10^-4 to 10^-2 M, and the sensor could be used for more than 3 months.

Original language	English
Pages (from-to)	321-327
Number of pages	7
Journal	Analytica Chimica Acta
Volume	289
Issue number	3
DOIs	https://doi.org/10.1016/0003-2670(94)90008-X
Publication status	Published - 1994 May 10

Keywords

Avidin
Biotin
Electrodeposition
Enzymatic methods
Glucose oxidase
Immobilization
Sensors

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

Access to Document

10.1016/0003-2670(94)90008-X

Cite this

@article{546615e10d5f4dd99a482188cb553e3f,

title = "Electrochemical deposition of avidin on the surface of a platinum electrode for enzyme sensor applications",

abstract = "Avidin was electrochemically deposited on a platinum electrode without loss of binding activity to biotin, and biotin-labeled glucose oxidase was immobilized on the electrode through avidin-biotin binding. The electrodes thus prepared displayed excellent properties as a glucose sensor. The oxidation current is proportional to glucose concentration from 10-4 to 10-2 M, and the sensor could be used for more than 3 months.",

keywords = "Avidin, Biotin, Electrodeposition, Enzymatic methods, Glucose oxidase, Immobilization, Sensors",

author = "Tomonori Hoshi and Junichi Anzai and Tetsuo Osa",

year = "1994",

month = may,

day = "10",

doi = "10.1016/0003-2670(94)90008-X",

language = "English",

volume = "289",

pages = "321--327",

journal = "Analytica Chimica Acta",

issn = "0003-2670",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Electrochemical deposition of avidin on the surface of a platinum electrode for enzyme sensor applications

AU - Hoshi, Tomonori

AU - Anzai, Junichi

AU - Osa, Tetsuo

PY - 1994/5/10

Y1 - 1994/5/10

N2 - Avidin was electrochemically deposited on a platinum electrode without loss of binding activity to biotin, and biotin-labeled glucose oxidase was immobilized on the electrode through avidin-biotin binding. The electrodes thus prepared displayed excellent properties as a glucose sensor. The oxidation current is proportional to glucose concentration from 10-4 to 10-2 M, and the sensor could be used for more than 3 months.

AB - Avidin was electrochemically deposited on a platinum electrode without loss of binding activity to biotin, and biotin-labeled glucose oxidase was immobilized on the electrode through avidin-biotin binding. The electrodes thus prepared displayed excellent properties as a glucose sensor. The oxidation current is proportional to glucose concentration from 10-4 to 10-2 M, and the sensor could be used for more than 3 months.

KW - Avidin

KW - Biotin

KW - Electrodeposition

KW - Enzymatic methods

KW - Glucose oxidase

KW - Immobilization

KW - Sensors

UR - http://www.scopus.com/inward/record.url?scp=0028212879&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028212879&partnerID=8YFLogxK

U2 - 10.1016/0003-2670(94)90008-X

DO - 10.1016/0003-2670(94)90008-X

M3 - Article

AN - SCOPUS:0028212879

VL - 289

SP - 321

EP - 327

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

IS - 3

ER -

Electrochemical deposition of avidin on the surface of a platinum electrode for enzyme sensor applications

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this