Electrochemical deposition of avidin on the surface of a platinum electrode for enzyme sensor applications

Tomonori Hoshi; Junichi Anzai; Tetsuo Osa

doi:10.1016/0003-2670(94)90008-X

Electrochemical deposition of avidin on the surface of a platinum electrode for enzyme sensor applications

Tomonori Hoshi, Junichi Anzai, Tetsuo Osa

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Avidin was electrochemically deposited on a platinum electrode without loss of binding activity to biotin, and biotin-labeled glucose oxidase was immobilized on the electrode through avidin-biotin binding. The electrodes thus prepared displayed excellent properties as a glucose sensor. The oxidation current is proportional to glucose concentration from 10^-4 to 10^-2 M, and the sensor could be used for more than 3 months.

Original language	English
Pages (from-to)	321-327
Number of pages	7
Journal	Analytica Chimica Acta
Volume	289
Issue number	3
DOIs	https://doi.org/10.1016/0003-2670(94)90008-X
Publication status	Published - 1994 May 10

Keywords

Avidin
Biotin
Electrodeposition
Enzymatic methods
Glucose oxidase
Immobilization
Sensors

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

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10.1016/0003-2670(94)90008-X

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abstract = "Avidin was electrochemically deposited on a platinum electrode without loss of binding activity to biotin, and biotin-labeled glucose oxidase was immobilized on the electrode through avidin-biotin binding. The electrodes thus prepared displayed excellent properties as a glucose sensor. The oxidation current is proportional to glucose concentration from 10-4 to 10-2 M, and the sensor could be used for more than 3 months.",

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AU - Hoshi, Tomonori

AU - Anzai, Junichi

AU - Osa, Tetsuo

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Y1 - 1994/5/10

N2 - Avidin was electrochemically deposited on a platinum electrode without loss of binding activity to biotin, and biotin-labeled glucose oxidase was immobilized on the electrode through avidin-biotin binding. The electrodes thus prepared displayed excellent properties as a glucose sensor. The oxidation current is proportional to glucose concentration from 10-4 to 10-2 M, and the sensor could be used for more than 3 months.

AB - Avidin was electrochemically deposited on a platinum electrode without loss of binding activity to biotin, and biotin-labeled glucose oxidase was immobilized on the electrode through avidin-biotin binding. The electrodes thus prepared displayed excellent properties as a glucose sensor. The oxidation current is proportional to glucose concentration from 10-4 to 10-2 M, and the sensor could be used for more than 3 months.

KW - Avidin

KW - Biotin

KW - Electrodeposition

KW - Enzymatic methods

KW - Glucose oxidase

KW - Immobilization

KW - Sensors

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IS - 3

ER -

Electrochemical deposition of avidin on the surface of a platinum electrode for enzyme sensor applications

Abstract

Keywords

ASJC Scopus subject areas

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