Electrochemical characterization of enzyme and immunoglobulin G patterned using microcontact printing

Hitoshi Shiku, Ayako Kumagai, Hong Qun Luo, Yasufumi Takahashi, Tomoyuki Yasukawa, Hiroshi Yamada, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Microcontact printing (μCP) with various proteins has been widely applied to biosensors and cell biology research. However, the mechanism of protein patterning in the μCP process is not clear in detail We have electrochemically estimated enzyme concentration on glass slide patterned with μCP technique. We also estimated the enzyme concentration at the surface of poly(dimethylsiloxane) (PDMS), which corresponded to the enzyme activity at the PDMS stamp just before μCP. Our result suggested that it was possible to transfer enzyme monolayer from PDMS to glass surfaces with 100% efficiency with μCP. Immunoglobulin G (IgG) patterned with μCP was also characterized by tagging with enzyme labeled anti-IgG. Scanning electrochemical microscopy (SECM) image was obtained to visualize line and space pattern of IgG monolayer.

Original languageEnglish
Pages (from-to)122-125
Number of pages4
JournalElectrochemistry
Volume78
Issue number2
DOIs
Publication statusPublished - 2010 Feb

Keywords

  • Beta-galactosidase
  • Diaphorase
  • Microcontact printing
  • Scanning electrochemical microscopy

ASJC Scopus subject areas

  • Electrochemistry

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