Electrochemical characterization of enzymatic activity of yeast cells entrapped in a poly(dimethylsiloxane) microwell on the basis of limited diffusion system

Hitoshi Shiku, Shun Goto, Sungbong Jung, Kuniaki Nagamine, Masahiro Koide, Tomosato Itayama, Tomoyuki Yasukawa, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

A highly sensitive and quantitative analysis was performed using a poly(dimethylsiloxane) (PDMS) microwell array in a scanning electrochemical microscopy setup. A microelectrode with a relatively large seal radius was used to cover the top of the cylindrical PDMS microwell (96 pL). The voltammogram for 4 mM ferrocyanide resulted in a charge value of 38 nC, suggesting that almost 100% of the reductant in the microwell was converted to the oxidation current. When genetically modified yeast cells were entrapped in the microwell, the accumulation of p-aminophenol (PAP) produced by expressing β-galactosidase (βGAL) was successfully observed.

Original languageEnglish
Pages (from-to)182-187
Number of pages6
JournalAnalyst
Volume134
Issue number1
DOIs
Publication statusPublished - 2009 Jan 1

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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