Electrochemical biosensor based on protein-polysaccharide hybrid for selective detection of nanomolar dopamine metabolite of 3,4- dihydroxyphenylacetic acid (DOPAC)

Aihua Liu, Itaru Honma, Haoshen Zhou

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

A strategy for the preparation of tyrosinase biosensor has been proposed to determine dopamine metabolite of 3,4-dihydroxyphenylacetic acid (DOPAC). An amino-polysaccharide chitosan was successfully used as matrix to entrap tyrosinase on glassy carbon electrode surface on basis of tyrosinase conjugated with chitosan. The sensor was operated at -0.15 V. The current linearly increased with the increasing concentration of DOPAC over the concentration of 6 nM to 0.2 mM. The sensor can be used to selectively determine nanomolar DOPAC in the presence of physiological level of ascorbic acid (AA), uric acid (UA), acetaminophen, neurotransmitters including dopamine, l-dopa, adrenaline and noradrenaline.

Original languageEnglish
Pages (from-to)233-236
Number of pages4
JournalElectrochemistry Communications
Volume7
Issue number2
DOIs
Publication statusPublished - 2005 Feb 1
Externally publishedYes

Keywords

  • Biosensor
  • Chitosan
  • DOPAC
  • Neurotransmitters
  • Tyrosinase

ASJC Scopus subject areas

  • Electrochemistry

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