We have developed a novel technique, Electrochemical Biolithography, which enables in situ cellular micropatterning by electrochemical means. The cytophobic feature of the albumin-coated substrate was found to be switched to cell-adhesive by dilute HBrO electrogenerated at the tip of Pt-microelectrode approached closely to the substrates surface. Our patterning technique using the microelectrode is applicable to a pre-cell-patterned surface and thus enables the multi-micropatterning with different types of cell populations. Also the in situ control of cell growth and migration has been achieved. In addition, the technique is simple enough to be integrated to the small and closed systems such as microfluidic devices. The resulting cellular micropatterns on a single cell level have been characterized by electrochemical and photochemical means as a pharmacological model of cellular networks.
|Number of pages||7|
|Publication status||Published - 2005 Dec 13|
|Event||Nanoscale Devices, Materials, and Biological Systems: Fundamental and Applications - Proceedings of the International Symposium - Honolulu, HI, United States|
Duration: 2004 Oct 3 → 2004 Oct 8
|Other||Nanoscale Devices, Materials, and Biological Systems: Fundamental and Applications - Proceedings of the International Symposium|
|Period||04/10/3 → 04/10/8|
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