Abstract
A novel hydrogen bond-forming ligand for cytosine-related single nucleotide polymorphism, which contains both a fluorescent naphthyridine moiety and a ferrocene group as an electrochemical indicator, is described. Hydrogen bond-mediated recognition for a target nucleobase within an abasic site-containing DNA duplex was confirmed by both fluorescence and electrochemical measurements. The analysis by fluorescence titration reveals that the ligand shows significant fluorescent quenching upon formation of a 1: 1 complex with the target nucleobase opposite an AP site, and the selectivity was in the order of cytosine > thymine > adenine, guanine, reflecting the stability of hydrogen bond formation.
Original language | English |
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Pages (from-to) | 295-296 |
Number of pages | 2 |
Journal | Nucleic acids symposium series (2004) |
Issue number | 51 |
DOIs | |
Publication status | Published - 2007 |
ASJC Scopus subject areas
- Medicine(all)