Electrochemical and fluorescence detection of cytosine-related SNPs using a ferrocenyl naphthyridine derivative.

Kotaro Morita, Yusuke Sato, Takehiro Seino, Seiichi Nishizawa, Norio Teramae

Research output: Contribution to journalArticlepeer-review

Abstract

A novel hydrogen bond-forming ligand for cytosine-related single nucleotide polymorphism, which contains both a fluorescent naphthyridine moiety and a ferrocene group as an electrochemical indicator, is described. Hydrogen bond-mediated recognition for a target nucleobase within an abasic site-containing DNA duplex was confirmed by both fluorescence and electrochemical measurements. The analysis by fluorescence titration reveals that the ligand shows significant fluorescent quenching upon formation of a 1: 1 complex with the target nucleobase opposite an AP site, and the selectivity was in the order of cytosine > thymine > adenine, guanine, reflecting the stability of hydrogen bond formation.

Original languageEnglish
Pages (from-to)295-296
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number51
DOIs
Publication statusPublished - 2007

ASJC Scopus subject areas

  • Medicine(all)

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