Electrochemical activity imaging of enzymes immobilized on substrates based on a Bio-LSI system

Toshiki Hokuto; Tomoyuki Yasukawa; Ryota Kunikata; Atsushi Suda; Kumi Y. Inoue; Tomokazu Matsue; Fumio Mizutani

doi:10.1246/cl.140055

Electrochemical activity imaging of enzymes immobilized on substrates based on a Bio-LSI system

Toshiki Hokuto, Tomoyuki Yasukawa, Ryota Kunikata, Atsushi Suda, Kumi Y. Inoue, Tomokazu Matsue, Fumio Mizutani

ENV - Frontier Science for Advanced Environment

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The activities of glucose oxidase (GOx) and lactate oxidase (LOx) immobilized on a substrate were obtained as images of oxidation currents of H₂O₂ using a Bio-LSI system. In the presence of appropriate substrates, the image of the enzyme appeared as lines with a high oxidation current. The time required to obtain the current image was 10 s, a significant decrease from the time required by the scanning method with a miniaturized probe.

Original language	English
Pages (from-to)	758-759
Number of pages	2
Journal	Chemistry Letters
Volume	43
Issue number	6
DOIs	https://doi.org/10.1246/cl.140055
Publication status	Published - 2014

ASJC Scopus subject areas

Chemistry(all)

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10.1246/cl.140055

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title = "Electrochemical activity imaging of enzymes immobilized on substrates based on a Bio-LSI system",

abstract = "The activities of glucose oxidase (GOx) and lactate oxidase (LOx) immobilized on a substrate were obtained as images of oxidation currents of H2O2 using a Bio-LSI system. In the presence of appropriate substrates, the image of the enzyme appeared as lines with a high oxidation current. The time required to obtain the current image was 10 s, a significant decrease from the time required by the scanning method with a miniaturized probe.",

author = "Toshiki Hokuto and Tomoyuki Yasukawa and Ryota Kunikata and Atsushi Suda and Inoue, {Kumi Y.} and Tomokazu Matsue and Fumio Mizutani",

year = "2014",

doi = "10.1246/cl.140055",

language = "English",

volume = "43",

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journal = "Chemistry Letters",

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publisher = "Chemical Society of Japan",

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TY - JOUR

T1 - Electrochemical activity imaging of enzymes immobilized on substrates based on a Bio-LSI system

AU - Hokuto, Toshiki

AU - Yasukawa, Tomoyuki

AU - Kunikata, Ryota

AU - Suda, Atsushi

AU - Inoue, Kumi Y.

AU - Matsue, Tomokazu

AU - Mizutani, Fumio

PY - 2014

Y1 - 2014

N2 - The activities of glucose oxidase (GOx) and lactate oxidase (LOx) immobilized on a substrate were obtained as images of oxidation currents of H2O2 using a Bio-LSI system. In the presence of appropriate substrates, the image of the enzyme appeared as lines with a high oxidation current. The time required to obtain the current image was 10 s, a significant decrease from the time required by the scanning method with a miniaturized probe.

AB - The activities of glucose oxidase (GOx) and lactate oxidase (LOx) immobilized on a substrate were obtained as images of oxidation currents of H2O2 using a Bio-LSI system. In the presence of appropriate substrates, the image of the enzyme appeared as lines with a high oxidation current. The time required to obtain the current image was 10 s, a significant decrease from the time required by the scanning method with a miniaturized probe.

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U2 - 10.1246/cl.140055

DO - 10.1246/cl.140055

M3 - Article

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VL - 43

SP - 758

EP - 759

JO - Chemistry Letters

JF - Chemistry Letters

IS - 6

ER -

Electrochemical activity imaging of enzymes immobilized on substrates based on a Bio-LSI system

Abstract

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