Electrically induced contraction of C2C12 myotubes cultured on a porous membrane-based substrate with muscle tissue-like stiffness

Hirokazu Kaji, Takeshi Ishibashi, Kuniaki Nagamine, Makoto Kanzaki, Matsuhiko Nishizawa

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)


A porous membrane-based cell culture device was developed to electrically stimulate a confluent monolayer of C2C12 myotubes. The device's cell culture substrate is a microporous alumina membrane-modified by attaching an atelocollagen membrane on the upperside and a hole-spotted poly(dimethylsiloxane) (PDMS) film on the underside. When electric current is generated between the device's Pt ring electrodes - one of which is placed above the cells and the other below the PDMS layer - the focused current at the PDMS hole can electrically stimulate the cells. C2C12 myoblasts were cultured on the substrate and differentiated into myotubes. When the electrical pulses were applied, myotubes started to contract slightly in and near the hole, and that the continuous stimulation increased both the number of stimuli-responding myotubes and the magnitude of the contraction considerably owing to the underlying atelocollagen membrane with muscle tissue-like stiffness. Also, the generation of contractile myotubes on a wider region of the membrane substrate was possible by applying the electrical pulses through the array of holes in the PDMS film. Using the present system, the glucose uptake by contractile myotubes was examined with fluorescence-labeled glucose, 2-NBDG, which displayed a positive correlation between the contractile activity of myotubes and the uptake of 2-NBDG.

Original languageEnglish
Pages (from-to)6981-6986
Number of pages6
Issue number27
Publication statusPublished - 2010 Sep


  • Atelocollagen membrane
  • C2C12 myotube
  • Contractility
  • Electrical pulse stimulation
  • Glucose uptake

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials


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