Electric-field-induced changes in absorption and fluorescence of the green fluorescent protein chromophore in a PMMA film

Takakazu Nakabayashi, Kazuyuki Hino, Yuka Ohta, Sayuri Ito, Hirofumi Nakano, Nobuhiro Ohta

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

External electric field effects on absorption, fluorescence, and fluorescence decay of p-HBDI that is a model compound of the chromophore of GFP have been examined in a poly(methyl methacrylate) film. The electroabsorption spectrum is similar in shape to the first derivative of the absorption spectrum, which results from the difference in molecular polarizability between the ground state and the Franck-Condon excited state. The electrophotoluminescence spectrum is dominated by the corresponding fluorescence spectrum, indicating the enhancement of the fluorescence intensity in the presence of external electric fields. The direct measurements of the electric field effect on the fluorescence decay profile suggest that the field-induced deceleration of the nonradiative process contributes to the increase in the fluorescence intensity in the presence of electric fields.

Original languageEnglish
Pages (from-to)8622-8626
Number of pages5
JournalJournal of Physical Chemistry B
Volume115
Issue number26
DOIs
Publication statusPublished - 2011 Jul 7
Externally publishedYes

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Fingerprint Dive into the research topics of 'Electric-field-induced changes in absorption and fluorescence of the green fluorescent protein chromophore in a PMMA film'. Together they form a unique fingerprint.

Cite this