Electric field effects on fluorescence of the green fluorescent protein

Takakazu Nakabayashi, Masataka Kinjo, Nobuhiro Ohta

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

External electric field effects on state energy and photoexcitation dynamics have been examined for a mutant of UV-excited green fluorescent protein (GFPuv5) in a PVA film. The electrofluorescence spectrum of GFPuv5 is reproduced by a linear combination between the fluorescence spectrum and its second derivative spectrum, indicating the field-induced fluorescence quenching and the difference in electric dipole moment between the fluorescent state and the ground state. The direct measurements of the field-induced change in fluorescence decay show that the field-induced quenching results from the field-induced increase in the rate of the non-radiative process from the fluorescent state.

Original languageEnglish
Pages (from-to)408-412
Number of pages5
JournalChemical Physics Letters
Volume457
Issue number4-6
DOIs
Publication statusPublished - 2008 May 27
Externally publishedYes

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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