Elcrochemical bio-lithography for in-situ immobilization of proteins and cells within microchannels

Matsuhiko Nishizawa, Hirokazu Kaji, Kazuto Tsukidate, Masahiko Hashimoto, Takashi Abe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The use of microfluidic system is a recent trend in diagnostic assays and bioreactors based on the protein and cellular engineering. Most of microfluidic biodevices would require the combination with a technique to pattern proteins and living cells within microchannels just prior to the use of the devices, since these bio-elements are delicate against desiccation, oxidation and heat. Here, we present a simple, electrochemical method to locally immobilize proteins and cells on substrate surface, easily applicable to the microfluidic system. The integration of the technique into a microfluidic device and the on-demand immobilization of proteins and cells were achieved. The sandwich immunoassay within the microchannel was demonstrated, in which a series of protocols from immobilization to measurement can be carried out under physiochemical conditions.

Original languageEnglish
Title of host publicationTRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers
Pages151-154
Number of pages4
DOIs
Publication statusPublished - 2005 Nov 9
Event13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05 - Seoul, Korea, Republic of
Duration: 2005 Jun 52005 Jun 9

Publication series

NameDigest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05
Volume1

Other

Other13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05
CountryKorea, Republic of
CitySeoul
Period05/6/505/6/9

Keywords

  • Lithography
  • Microchannel
  • Microelectrode
  • Micropatterning
  • Protein adsorption

ASJC Scopus subject areas

  • Engineering(all)

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    Nishizawa, M., Kaji, H., Tsukidate, K., Hashimoto, M., & Abe, T. (2005). Elcrochemical bio-lithography for in-situ immobilization of proteins and cells within microchannels. In TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers (pp. 151-154). [1D4.3] (Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05; Vol. 1). https://doi.org/10.1109/SENSOR.2005.1496381