Elastic modulus of suspended purple membrane measured by atomic force microscopy

Ari M. Siitonen; Koji Sumitomo; Chandra S. Ramanujan; Youichi Shinozaki; Nahoko Kasai; Kazuaki Furukawa; John F. Ryan; Keiichi Torimitsu

doi:10.1016/j.apsusc.2008.03.009

Elastic modulus of suspended purple membrane measured by atomic force microscopy

Ari M. Siitonen, Koji Sumitomo, Chandra S. Ramanujan, Youichi Shinozaki, Nahoko Kasai, Kazuaki Furukawa, John F. Ryan, Keiichi Torimitsu

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We have probed the mechanical properties of purple membrane (PM) in a physiological environment using the atomic force microscope (AFM). By suspending PM over nano-trenches, the elastic properties of PM can be evaluated free from the interaction with the substrate. Force-displacement curves were obtained on the suspended membrane and the data was compared to that of a simple model of a thin film over a trench. By fitting the data to the model, the elastic modulus of PM was estimated to be 8 MPa. When the membrane is repeatedly indented, we observed a change in the force-distance data consistent with damage to the two-dimensional crystal of PM. In this paper we demonstrate that the AFM allows us to evaluate the mechanics of biological membranes in their native conditions.

Original language	English
Pages (from-to)	7877-7880
Number of pages	4
Journal	Applied Surface Science
Volume	254
Issue number	23
DOIs	https://doi.org/10.1016/j.apsusc.2008.03.009
Publication status	Published - 2008 Sep 30
Externally published	Yes

Keywords

Elastic modulus
Membrane protein
Nanobiotechnology
Scanning probe microscopy

ASJC Scopus subject areas

Chemistry(all)
Condensed Matter Physics
Physics and Astronomy(all)
Surfaces and Interfaces
Surfaces, Coatings and Films

Access to Document

10.1016/j.apsusc.2008.03.009

Cite this

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title = "Elastic modulus of suspended purple membrane measured by atomic force microscopy",

abstract = "We have probed the mechanical properties of purple membrane (PM) in a physiological environment using the atomic force microscope (AFM). By suspending PM over nano-trenches, the elastic properties of PM can be evaluated free from the interaction with the substrate. Force-displacement curves were obtained on the suspended membrane and the data was compared to that of a simple model of a thin film over a trench. By fitting the data to the model, the elastic modulus of PM was estimated to be 8 MPa. When the membrane is repeatedly indented, we observed a change in the force-distance data consistent with damage to the two-dimensional crystal of PM. In this paper we demonstrate that the AFM allows us to evaluate the mechanics of biological membranes in their native conditions.",

keywords = "Elastic modulus, Membrane protein, Nanobiotechnology, Scanning probe microscopy",

author = "Siitonen, {Ari M.} and Koji Sumitomo and Ramanujan, {Chandra S.} and Youichi Shinozaki and Nahoko Kasai and Kazuaki Furukawa and Ryan, {John F.} and Keiichi Torimitsu",

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doi = "10.1016/j.apsusc.2008.03.009",

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T1 - Elastic modulus of suspended purple membrane measured by atomic force microscopy

AU - Siitonen, Ari M.

AU - Sumitomo, Koji

AU - Ramanujan, Chandra S.

AU - Shinozaki, Youichi

AU - Kasai, Nahoko

AU - Furukawa, Kazuaki

AU - Ryan, John F.

AU - Torimitsu, Keiichi

PY - 2008/9/30

Y1 - 2008/9/30

N2 - We have probed the mechanical properties of purple membrane (PM) in a physiological environment using the atomic force microscope (AFM). By suspending PM over nano-trenches, the elastic properties of PM can be evaluated free from the interaction with the substrate. Force-displacement curves were obtained on the suspended membrane and the data was compared to that of a simple model of a thin film over a trench. By fitting the data to the model, the elastic modulus of PM was estimated to be 8 MPa. When the membrane is repeatedly indented, we observed a change in the force-distance data consistent with damage to the two-dimensional crystal of PM. In this paper we demonstrate that the AFM allows us to evaluate the mechanics of biological membranes in their native conditions.

AB - We have probed the mechanical properties of purple membrane (PM) in a physiological environment using the atomic force microscope (AFM). By suspending PM over nano-trenches, the elastic properties of PM can be evaluated free from the interaction with the substrate. Force-displacement curves were obtained on the suspended membrane and the data was compared to that of a simple model of a thin film over a trench. By fitting the data to the model, the elastic modulus of PM was estimated to be 8 MPa. When the membrane is repeatedly indented, we observed a change in the force-distance data consistent with damage to the two-dimensional crystal of PM. In this paper we demonstrate that the AFM allows us to evaluate the mechanics of biological membranes in their native conditions.

KW - Elastic modulus

KW - Membrane protein

KW - Nanobiotechnology

KW - Scanning probe microscopy

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JO - Applied Surface Science

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Elastic modulus of suspended purple membrane measured by atomic force microscopy

Abstract

Keywords

ASJC Scopus subject areas

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