Efflux pump inhibitors reduce the invasiveness of Pseudomonas aeruginosa

Yoichi Hirakata, Akira Kondo, Kazuki Hoshino, Hisakazu Yano, Kazuaki Arai, Ayako Hirotani, Hiroyuki Kunishima, Natsuo Yamamoto, Masumitsu Hatta, Miho Kitagawa, Shigeru Kohno, Mitsuo Kaku

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Efflux systems are thought to contribute to antimicrobial resistance in Pseudomonas aeruginosa. The mexAB-oprM deletion strain of P. aeruginosa PAO1 is compromised in its capacity to invade Madin-Darby canine kidney (MDCK) cells, suggesting that P. aeruginosa exports invasion determinants using a MexAB-OprM system. The influences of efflux pump inhibitors (EPIs), including the broad-spectrum EPI Phe-Arg-β-naphthylamide (PAβN) and MexAB-OprM-specific EPI D13-9001, on the invasion of wild-type (WT) P. aeruginosa PAO1 and its MexAB-OprM-overproducing nalB strain were examined. The invasiveness of PAO1 WT and nalB strains was inhibited in the presence of EPIs in a concentration-dependent manner. Reduction of the invasiveness of both strains was greater for D13-9001 compared with PAβN. EPIs are thought to be useful in reducing the invasiveness and antimicrobial resistance of P. aeruginosa and thus may be promising as new anti-infectious agents.

Original languageEnglish
Pages (from-to)343-346
Number of pages4
JournalInternational Journal of Antimicrobial Agents
Volume34
Issue number4
DOIs
Publication statusPublished - 2009 Oct

Keywords

  • Efflux pump inhibitors
  • Invasiveness
  • MDCK cell
  • New anti-infectious agent
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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