Efficient repair of DNA damage induced by heavy ion particles in meiotic prophase I nuclei of Caenorhabditis elegans

Takako Takanami, Yongzhao Zhang, Hidetoshi Aoki, Tomoko Abe, Shigeo Yoshida, Hideyuki Takahashi, Saburo Horiuchi, Atsushi Higashitani

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The effects of heavy ion particle irradiation on meiosis and reproductive development in the nematode Caenorhabditis elegans were studied. Meiotic pachytene nuclei are significantly resistant to particle irradiation by the heavy ions carbon and argon, as well as to X-rays, but not UV, whereas diplotene to diakinesis stage oocytes and early embryonic cells are not. Chromosomal abnormalities appear in mitotic cells and in maturing oocytes irradiated with heavy ion particles during the diplotene to the early diakinesis stages, but not in oocytes irradiated during the pachytene stage. The pachytene nuclei of ced-3 mutants, which are defective in apoptosis, are similarly resistant to ionizing radiation, but pachytene nuclei depleted for Ce-atl-1 (ataxia-telangiectasia like 1) or Ce-rdh-1/rad-51 are more sensitive. Pachytene nuclei thus appear to effectively repair heavy ion-induced DNA damage by the meiotic homologous recombination system.

Original languageEnglish
Pages (from-to)271-276
Number of pages6
JournalJournal of radiation research
Volume44
Issue number3
DOIs
Publication statusPublished - 2003 Sep

Keywords

  • Heavy ion particle
  • Homologous recombination
  • Meiosis
  • Pachytene
  • RNAi

ASJC Scopus subject areas

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Health, Toxicology and Mutagenesis

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