Efficient production of celery embryos and plantlets released in culture of immobilized gel beads

Ken Ichiro Suehara, Katsuhito Kohketsu, Nobuyuki Uozumi, Takeshi Kobayashi

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Celery embryos and plantlets were found to be selectively released in a culture of immobilized Ca-alginate gel beads in which celery callus was entrapped under regeneration conditions. We studied the feasibility of use of this process for celery embryogenesis in an artificial seed system. The cells released from the gel beads were larger than those obtained in suspension culture. The optimal concentration of alginate gel for embryo and plantlet production was 2% for the immobilized cell culture. Considering the maintenance of the gel bead structure and detrimental effect of CaCl2 on plantlet development, 5 mM CaCl2 supplementation gave the best result in terms of the number of heart and torpedo embryos and plantlets. The ratio of the number of heart embryos, torpedo embryos and plantlets to total number of cells in the immobilized cell culture was higher than that in the suspension culture. Repeated batch culture with 5 mM CaCl2 provided long-term (more than 154 d) embryo and plantlet production without gel beads disruption. Productivity of plantlets in the immobilized cell culture with 5 mM CaCl2 was 2.2-fold as high as that in the suspension culture.

Original languageEnglish
Pages (from-to)585-588
Number of pages4
JournalJournal of Fermentation and Bioengineering
Volume79
Issue number6
DOIs
Publication statusPublished - 1995
Externally publishedYes

Keywords

  • alginate gel bead
  • artificial seed
  • immobilization
  • plant regeneration
  • somatic embryogenesis

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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