TY - JOUR
T1 - Efficient mutation induction using heavy-ion beam irradiation and simple genomic screening with random primers in taro (Colocasia esculenta L. Schott)
AU - Matsuyama, Tomoki
AU - Watanabe, Manabu
AU - Murota, Yuri
AU - Nakata, Nanako
AU - Kitamura, Hisashi
AU - Shimokawa, Takashi
AU - Ebisuzaki, Toshikazu
AU - Wada, Satoshi
AU - Sato, Shusei
AU - Tabata, Satoshi
N1 - Funding Information:
This work was supported by Grants-in-Aid Research and Development Projects for Application in Promoting New Policy of the Ministry of Agriculture, Forestry and Fisheries of Japan [grant number 22038 ] and Research Project with Heavy Ions at NIRS-HIMAC [ 11J277 ].
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/10/15
Y1 - 2020/10/15
N2 - Progress in conventional breeding methods for taro (Colocasia esculenta L. Schott) via crossing has been limited, and suitable genetic materials for the development of new cultivars are scarce as most commercial taro cultivars are either non-flowering or rarely flowering triploids. In an attempt to advance taro breeding, we performed mutational breeding by heavy-ion beam irradiation of multiple shoots of ‘Chiba maru’ cultivar. Using 2–10 Gy neon and carbon ion beams, we achieved a plant survival rate of more than 90 % and used 94 surviving plants for genomic screening. To efficiently detect DNA polymorphisms induced by ion beam irradiation in young plants, we used five sets of 15-mer randomly amplified polymorphic DNA and arbitrarily primed-polymerase chain reaction random primers based on retrotransposon sequences for genomic screening. Two plants had polymorphic DNA bands, and the specific DNA patterns were maintained in all leaves. In one of these plants, which lacked somatic mosaicism (Cm10), the polymorphic patterns were maintained in the leaves and cormels of clones propagated from daughter cormels. Ion beam irradiation of multiple taro shoots could thus generate mutants that can be developed as new cultivars; the resulting novel polymorphic patterns would facilitate inter- and intra-cultivar identification.
AB - Progress in conventional breeding methods for taro (Colocasia esculenta L. Schott) via crossing has been limited, and suitable genetic materials for the development of new cultivars are scarce as most commercial taro cultivars are either non-flowering or rarely flowering triploids. In an attempt to advance taro breeding, we performed mutational breeding by heavy-ion beam irradiation of multiple shoots of ‘Chiba maru’ cultivar. Using 2–10 Gy neon and carbon ion beams, we achieved a plant survival rate of more than 90 % and used 94 surviving plants for genomic screening. To efficiently detect DNA polymorphisms induced by ion beam irradiation in young plants, we used five sets of 15-mer randomly amplified polymorphic DNA and arbitrarily primed-polymerase chain reaction random primers based on retrotransposon sequences for genomic screening. Two plants had polymorphic DNA bands, and the specific DNA patterns were maintained in all leaves. In one of these plants, which lacked somatic mosaicism (Cm10), the polymorphic patterns were maintained in the leaves and cormels of clones propagated from daughter cormels. Ion beam irradiation of multiple taro shoots could thus generate mutants that can be developed as new cultivars; the resulting novel polymorphic patterns would facilitate inter- and intra-cultivar identification.
KW - Colocasia esculenta
KW - DNA marker
KW - Genomic screening
KW - Heavy-ion beam irradiation
KW - Mutational breeding
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U2 - 10.1016/j.scienta.2020.109568
DO - 10.1016/j.scienta.2020.109568
M3 - Article
AN - SCOPUS:85087128008
VL - 272
JO - Scientia Horticulturae
JF - Scientia Horticulturae
SN - 0304-4238
M1 - 109568
ER -