Abstract
Isolation of human parainfluenza virus (HPIV) serotypes 1 and 3 from clinical specimens is not very efficient because of the lack of a cell culture system capable of inducing CPE. In this study, the utility of a melanoma cell line, MNT-1, that allows HPIV growth and displays CPE was demonstrated. In particularly, the efficiency of isolating HPIV1 and HPIV3 using MNT-1 was greater than for cell lines conventionally used for HPIV isolation. Our demonstrated efficacy of HPIV1 and HPIV3 isolation with apparent CPE using the MNT-1 cell culture system has the potential to improve virus isolation from clinical specimens.
Original language | English |
---|---|
Pages (from-to) | 801-805 |
Number of pages | 5 |
Journal | MICROBIOLOGY and IMMUNOLOGY |
Volume | 60 |
Issue number | 11 |
DOIs | |
Publication status | Published - 2016 Nov 1 |
Keywords
- MNT-1 cells
- cytopathic effect
- human parainfluenza virus
- virus isolation
ASJC Scopus subject areas
- Microbiology
- Immunology
- Virology