Efficient isolation of human parainfluenza viruses 1 and 3 using MNT-1, a human malignant melanoma cell line system that exhibits an apparent cytopathic effect

Ko Sato, Oshi Watanabe, Suguru Ohmiya, Fumiko Chiba, Masahiro Hayashi, Tamio Suzuki, Kazuyoshi Kawakami, Hidekazu Nishimura

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Isolation of human parainfluenza virus (HPIV) serotypes 1 and 3 from clinical specimens is not very efficient because of the lack of a cell culture system capable of inducing CPE. In this study, the utility of a melanoma cell line, MNT-1, that allows HPIV growth and displays CPE was demonstrated. In particularly, the efficiency of isolating HPIV1 and HPIV3 using MNT-1 was greater than for cell lines conventionally used for HPIV isolation. Our demonstrated efficacy of HPIV1 and HPIV3 isolation with apparent CPE using the MNT-1 cell culture system has the potential to improve virus isolation from clinical specimens.

Original languageEnglish
Pages (from-to)801-805
Number of pages5
JournalMICROBIOLOGY and IMMUNOLOGY
Volume60
Issue number11
DOIs
Publication statusPublished - 2016 Nov 1

Keywords

  • MNT-1 cells
  • cytopathic effect
  • human parainfluenza virus
  • virus isolation

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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