TY - JOUR
T1 - Efficient immortalization of cells derived from critically endangered Tsushima leopard cat (Prionailurus bengalensis euptilurus) with expression of mutant CDK4, Cyclin D1, and telomerase reverse transcriptase
AU - Gouko, Ryo
AU - Onuma, Manabu
AU - Eitsuka, Takahiro
AU - Katayama, Masafumi
AU - Takahashi, Kouhei
AU - Nakagawa, Kiyotaka
AU - Inoue-Murayama, Miho
AU - Kiyono, Tohru
AU - Fukuda, Tomokazu
N1 - Funding Information:
Acknowledgement We thank Dr. Hiroyuki Miyoshi (RIKEN, BioResource Center) for providing lentiviral constructs. This work was supported by the Grant from JSPS, KAKENHI, Grant Number 17H03624.
Publisher Copyright:
© 2018, Springer Nature B.V.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Tsushima leopard cat is the subspecies of Amur cats, and it is classified as the most highest class of critically endangered animals. Although the protection activity is highly recognized, the number of animals is decreasing due to the human activity and invasion of domestic cats and infectious disease. In this study, we succeeded primary culture of normal fibroblasts derived from the Tsushima leopard cat (Prionailurus bengalensis euptilurus). Furthermore, we introduced the human derived mutant Cyclin Dependent Kinase 4, Cyclin D1, and telomere reverse transcriptase. We showed that the expression of these three genes efficiently immortalized cells derived from Tsushima leopard cat. Furthermore, we showed that the chromosome pattern of the established cells is identical with the original one. These data indicate that our method of immortalization is useful to establish cell lines from critically endangered cats, which potentially contributes to the re-generation of critically endangered animals from cultured cell with reproductive technique, such as somatic cloning.
AB - Tsushima leopard cat is the subspecies of Amur cats, and it is classified as the most highest class of critically endangered animals. Although the protection activity is highly recognized, the number of animals is decreasing due to the human activity and invasion of domestic cats and infectious disease. In this study, we succeeded primary culture of normal fibroblasts derived from the Tsushima leopard cat (Prionailurus bengalensis euptilurus). Furthermore, we introduced the human derived mutant Cyclin Dependent Kinase 4, Cyclin D1, and telomere reverse transcriptase. We showed that the expression of these three genes efficiently immortalized cells derived from Tsushima leopard cat. Furthermore, we showed that the chromosome pattern of the established cells is identical with the original one. These data indicate that our method of immortalization is useful to establish cell lines from critically endangered cats, which potentially contributes to the re-generation of critically endangered animals from cultured cell with reproductive technique, such as somatic cloning.
KW - Cellular senescence
KW - Endangered animals
KW - Immortalization
KW - Tsushima leopard cat
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U2 - 10.1007/s10616-018-0254-0
DO - 10.1007/s10616-018-0254-0
M3 - Article
AN - SCOPUS:85053611173
VL - 70
SP - 1619
EP - 1630
JO - Cytotechnology
JF - Cytotechnology
SN - 0920-9069
IS - 6
ER -