Efficient immortalization of cells derived from critically endangered Tsushima leopard cat (Prionailurus bengalensis euptilurus) with expression of mutant CDK4, Cyclin D1, and telomerase reverse transcriptase

Ryo Gouko, Manabu Onuma, Takahiro Eitsuka, Masafumi Katayama, Kouhei Takahashi, Kiyotaka Nakagawa, Miho Inoue-Murayama, Tohru Kiyono, Tomokazu Fukuda

    Research output: Contribution to journalArticlepeer-review

    13 Citations (Scopus)

    Abstract

    Tsushima leopard cat is the subspecies of Amur cats, and it is classified as the most highest class of critically endangered animals. Although the protection activity is highly recognized, the number of animals is decreasing due to the human activity and invasion of domestic cats and infectious disease. In this study, we succeeded primary culture of normal fibroblasts derived from the Tsushima leopard cat (Prionailurus bengalensis euptilurus). Furthermore, we introduced the human derived mutant Cyclin Dependent Kinase 4, Cyclin D1, and telomere reverse transcriptase. We showed that the expression of these three genes efficiently immortalized cells derived from Tsushima leopard cat. Furthermore, we showed that the chromosome pattern of the established cells is identical with the original one. These data indicate that our method of immortalization is useful to establish cell lines from critically endangered cats, which potentially contributes to the re-generation of critically endangered animals from cultured cell with reproductive technique, such as somatic cloning.

    Original languageEnglish
    Pages (from-to)1619-1630
    Number of pages12
    JournalCytotechnology
    Volume70
    Issue number6
    DOIs
    Publication statusPublished - 2018 Dec 1

    Keywords

    • Cellular senescence
    • Endangered animals
    • Immortalization
    • Tsushima leopard cat

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Biomedical Engineering
    • Clinical Biochemistry
    • Cell Biology

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