Efficient establishment of primary fibroblast cultures from the hawksbill sea turtle (Eretmochelys imbricata)

Tomokazu Fukuda, Jun Kurita, Tomomi Saito, Kei Yuasa, Masanobu Kurita, Kenichiro Donai, Hiroshi Nitto, Makoto Soichi, Katsuhiko Nishimori, Takafumi Uchida, Emiko Isogai, Manabu Onuma, Hideko Sone, Norihisa Oseko, Miho Inoue-Murayama

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The hawksbill sea turtle (Eretmochelys imbricata) is a critically endangered species at a risk of extinction. Preservation of the genomic and cellular information of endangered animals is important for future genetic and biological studies. Here, we report the efficient establishment of primary fibroblast cultures from skin tissue of the hawksbill sea turtle. We succeeded in establishing 19 primary cultures from 20 hawksbill sea turtle individuals (a success rate of 95%). These cells exhibited a fibroblast-like morphology and grew optimally at a temperature of 26 C, but experienced a loss of viability when cultured at 37 C. Chromosomal analysis using the primary cells derived here revealed that hawksbill sea turtles have a 2n = 56 karyotype. Furthermore, we showed that our primary cell cultures are free of several fish-related viruses, and this finding is important for preservation purposes. To our knowledge, this report is the first to describe primary cell cultures established from normal tissues of the hawksbill sea turtle. The results will contribute to the preservation of biodiversity, especially for the sea turtles that are critically endangered owing to human activities.

Original languageEnglish
Pages (from-to)660-665
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume48
Issue number10
DOIs
Publication statusPublished - 2012 Dec

Keywords

  • Chromosome analysis
  • Critically endangered animals
  • Cultured cells
  • Hawksbill sea turtle

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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