Efficient establishment of pig embryonic fibroblast cell lines with conditional expression of the simian vacuolating virus 40 large T fragment

Tomokazu Fukuda, Masafumi Katayama, Takayuki Yoshizawa, Takahiro Eitsuka, Hiroaki Mizukami, Kiyotaka Nakagawa, Hisao Ito, Homika Komagata, Sanghoun Song, Sanggun Roh, Yumi Hoshino, Eimei Sato, Hirofumi Hanada, Katsuhiko Nishimori, Teruo Miyazawa, Takafumi Uchida

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The pig is an important animal for both agricultural and medical purposes. However, the number of pig-derived cell lines is relatively limited when compared with mouse-and human-derived lines. We established in this study a retroviral conditional expression system for the Simian vacuolating virus 40 large T fragment (SV40T) which allowed us to efficiently establish pig embryonic fibroblast cell lines. The established cell lines showed high levels of cell proliferation and resistance to cellular senescence. A chromosome analysis showed that 84% of the cells had the normal karyotype. Transient expression of the Cre recombinase allowed us to excise the SV40T fragment from the genome. The development of this research tool will enable us to quickly establish new cell lines derived from various animals.

Original languageEnglish
Pages (from-to)1372-1377
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number7
DOIs
Publication statusPublished - 2012

Keywords

  • Conditional expression system
  • Immortalization
  • Retrovirus
  • Simian vacuolating virus 40 large T fragment (SV40T)

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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