Efficacy of amikacin combinations for nocardiosis

Keiji Kanemitsu, Hiroyuki Kunishima, Tomoo Saga, Hideo Harigae, Shiho Ishikawa, Hiromu Takemura, Mitsuo Kaku

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

We isolated five bacterial strains from patients diagnosed as having nocardiosis. Bacterial species were identified based on the similarities in the nucleotide sequences of 16S ribosomal RNAs. Three of the five strains were identified as Nocardia asteroids, but unexpectedly other two were Streptomyces hygroscopicus and Rothia dentocariosa. The latter two species are not members of the family Nocardiaceae. We investigated the susceptibilities of these five strains to the following nine antimicrobial agents: trimethoprim/sulfamethoxazole (TMP/SMX), minocycline (MINO), erythromycin (EM), amikacin (AMK), cefotaxime (CTX), faropenem (FRPM), imipenem (IPM), ciprofloxacin (CPFX), and sparfloxacin (SPFX). The minimum inhibitory concentration (MIC) ranges (mg/ml) were as follows: TMP-SMX, 4->32; MINO, 0.125-8; EM, &IE;0.016->32; AMK, 1-2; CTX, 0.063->32; FRPM, 0.063-16; IPM, 0.125-2; CPFX, 4-32; and SPFX, 0.5-16. Moreover, the synergistic effects of AMK in combination with each of TMP-SMX, MINO, EM, CTX, IPM, and SPFX were investigated by checkerboard synergy testing. No antagonism was recognized for the three N. asteroides strains. Synergistic and additive effects were observed for the combinations of AMK with CTX, IPM, or SPFX.

Original languageEnglish
Pages (from-to)157-163
Number of pages7
JournalTohoku Journal of Experimental Medicine
Volume201
Issue number3
DOIs
Publication statusPublished - 2003 Nov

Keywords

  • Amikacin
  • Combination therapy
  • FIC index
  • Nocardiosis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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