TY - JOUR
T1 - Effects of unilateral vagotomy on nitric oxide synthase and histamine H3 receptors in the rat dorsal vagal complex
AU - Zhao, Xiao Lan
AU - Yanai, Kazuhiko
AU - Hashimoto, Yasuhiko
AU - Steinbusch, Harry W.M.
AU - Watanabe, Takehiko
N1 - Funding Information:
We thank Drs. H. Esumi, H. Adachi and Y. Kurashima, National Cancer Center ResearchI nstitute, Chiba for kindly providing two antibodiest hat recognize neuronal and inducible NOS. We also thank Dr. David A. Hopkins at the Departmento f Anatomy and Neurobiology,F aculty of Medicine, Dalhousie University, Halifax, Canada, and Dr. Jong Hoon Ryu, Department of Toxicology, National Institute of Safety Research, Seoul, Korea, for valuable discussion. This work was supportedb y grants-in-aidfr om the Japanese Ministry of Education, Science, Sports and Culture ( # 05454663, # 05557008, # 06833002, # 07558107), the Uehara Memorial Foundation and SasagawaF oun-dation of Health Care.
PY - 1996/10
Y1 - 1996/10
N2 - Nitric oxide synthase (NOS) and histamine H3 receptors are both markedly increased by neuronal injuries. To examine whether peripheral axotomy produced differential changes in NOS and H3 receptors, both NOS and H3 receptors were measured in the dorsal vagal complex after unilateral vagotomy. The presence of NOS-positive neurons was examined using both NADPH-diaphorase histochemistry and neuronal NOS-immunohistochemistry in rats vagotomized at the mid-cervical level. NADPH-diaphorase activity and NOS-immunoreactivity were markedly enhanced on the dorsal motor nucleus of the vagus (DMX) and in the ambiguus nucleus at the denervated side. Intraperitoneal injection of NOS inhibitors, N(ω)-nitro-L-arginine (10 mg/kg) or dexamethasone (0.5 mg/kg) attenuated the increase in NADPH-diaphorase activity. Glial fibrillary acidic protein (GFAP) was similarly induced 2 weeks after vagotomy in the vagal complex and surrounding area. Histamine H3 receptors in the vagal complex were visualized with [3H]N(α)-methylhistamine. The ligand-labeled H3 receptors were mainly located at the nucleus of the solitary tract (NST). The densities of H3 receptors did not change in the NST after unilateral vagotomy. These results suggest that peripheral axotomy such as mid-cervical vagotomy preferentially induces NOS in damaged neurons without affecting the level of H3 receptors.
AB - Nitric oxide synthase (NOS) and histamine H3 receptors are both markedly increased by neuronal injuries. To examine whether peripheral axotomy produced differential changes in NOS and H3 receptors, both NOS and H3 receptors were measured in the dorsal vagal complex after unilateral vagotomy. The presence of NOS-positive neurons was examined using both NADPH-diaphorase histochemistry and neuronal NOS-immunohistochemistry in rats vagotomized at the mid-cervical level. NADPH-diaphorase activity and NOS-immunoreactivity were markedly enhanced on the dorsal motor nucleus of the vagus (DMX) and in the ambiguus nucleus at the denervated side. Intraperitoneal injection of NOS inhibitors, N(ω)-nitro-L-arginine (10 mg/kg) or dexamethasone (0.5 mg/kg) attenuated the increase in NADPH-diaphorase activity. Glial fibrillary acidic protein (GFAP) was similarly induced 2 weeks after vagotomy in the vagal complex and surrounding area. Histamine H3 receptors in the vagal complex were visualized with [3H]N(α)-methylhistamine. The ligand-labeled H3 receptors were mainly located at the nucleus of the solitary tract (NST). The densities of H3 receptors did not change in the NST after unilateral vagotomy. These results suggest that peripheral axotomy such as mid-cervical vagotomy preferentially induces NOS in damaged neurons without affecting the level of H3 receptors.
KW - Ambiguus nucleus
KW - Dorsal motor nucleus of vagus (DMX)
KW - Glial fibrillary acidic protein (GFAP)
KW - Histamine H receptors
KW - NADPH-diaphorase
KW - Nitric oxide synthase (NOS)
KW - Vagotomy
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U2 - 10.1016/S0891-0618(96)00163-9
DO - 10.1016/S0891-0618(96)00163-9
M3 - Article
C2 - 8951592
AN - SCOPUS:0030272648
VL - 11
SP - 221
EP - 229
JO - Journal of Chemical Neuroanatomy
JF - Journal of Chemical Neuroanatomy
SN - 0891-0618
IS - 4
ER -