Effects of unilateral phrenic nerve denervation on diaphragm contractility in rat.

C. Shindoh, W. Hida, H. Kurosawa, S. Ebihara, Y. Kikuchi, T. Takishima, K. Shirato

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

We examined the early effects of phrenic nerve denervation on the diaphragm muscle 1, 3, 7 and 14 days after unilateral denervation in rats. In the denervated hemidiaphragms, force frequency curves at 3, 7 and 14 days decreased significantly by 51%, 50% and 38% respectively of the peak tension of the force frequency curves of the diaphragms of rats with sham operation. Twitch tensions increased significantly at 14 days, and contraction times and half relaxation times slowed significantly at 3, 7 and 14 days. The tensions of denervated diaphragms at 5 min during the fatigue runs was significantly increased at 14 days. As determined by histological staining, the mean cross sectional area of fast-twitch fibers (type II) decreased significantly from 2,742 (sham) to 1,599 microns (14 days), but that of the slow-twitch fibers (type I) did not change significantly during the same period. These findings suggest that, during the first two weeks of denervation, fast twitch fibers (type II) atrophy more rapidly than slow twitch fatigue resistant fibers (type I), as confirmed by the contractile properties and histological findings.

Original languageEnglish
Pages (from-to)291-302
Number of pages12
Journalthe tohoku journal of experimental medicine
Volume173
Issue number3
DOIs
Publication statusPublished - 1994 Jul

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Effects of unilateral phrenic nerve denervation on diaphragm contractility in rat.'. Together they form a unique fingerprint.

Cite this