Effects of ultraviolet irradiation on genetical inactivation and morphological features of sperm of the Pacific oyster Crassostrea gigas

Q. I. Li, Makoto Osada, Masaru Kashihara, Ken Hirohashi, Akihiro Kijima

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Effects of ultraviolet (UV) irradiation on genetical inactivation and morphological features of sperm were examined in the Pacific oyster. Ultraviolet light (254 nm) was effective for inactivating sperm chromosomes. Irradiation for 60s at a UV intensity of 72erg/mm2 per s was the optimum dose to achieve haploid gynogenesis. The rates of the fertilization and the development of D-shaped larvae decreased with increasing irradiation time, and the development of the eggs fertilized with the genetically inactivated sperms terminated before reaching the D-shaped stage. Electron microscopy showed clear destruction of the sperm acrosome and flagellum in the UV-irradiated sperms. As the duration of irradiation increased, the acrosome of sperms tended to suffer greater damage until the sperms eventually lost their flagella. Abnormalities in these structures have appeared to account, at least in part, for the decline of the fertilization rate of eggs inseminated with UV-irradiated sperms.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalFisheries Science
Volume66
Issue number1
DOIs
Publication statusPublished - 2000 Jan 1

Keywords

  • Gynogenesis
  • Pacific oyster
  • Sperm morphology
  • Ultraviolet irradiation

ASJC Scopus subject areas

  • Aquatic Science

Fingerprint Dive into the research topics of 'Effects of ultraviolet irradiation on genetical inactivation and morphological features of sperm of the Pacific oyster Crassostrea gigas'. Together they form a unique fingerprint.

Cite this