Effects of ultraviolet irradiation on genetical inactivation and morphological structure of sperm of the Japanese scallop, Patinopecten yessoensis

Qi Li, Makoto Osada, Masaru Kashihara, Ken Hirohashi, Akihiro Kijima

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Effects of ultraviolet (UV) irradiation on genetic inactivation and morphological structure of sperms were examined in the scallop, Patinopecten yessoensis. Haploid gynogenesis of the scallop was successfully induced by 50-60 s UV irradiation of 720 μW cm-2 s-1. The fertilization rate apparently decreased with increasing irradiation time, and the development of the eggs fertilized with the genetically inactivated sperms terminated before reaching the D-shaped larvae stage. Scanning electron microscopy (SEM) showed clear destruction of the sperm acrosome and flagellum in the UV-irradiated sperms. As the duration of UV irradiation increased, the acrosome of sperms tended to suffer greater damage, until the sperms eventually lost their flagella. Abnormalities in these structures have appeared to account, at least in part, for the decline of the fertilization rate of eggs inseminated with UV-irradiated sperms. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)233-242
Number of pages10
JournalAquaculture
Volume186
Issue number3-4
DOIs
Publication statusPublished - 2000 Jun 15

Keywords

  • Gynogenesis
  • Japanese scallop
  • Sperm morphology
  • UV irradiation

ASJC Scopus subject areas

  • Aquatic Science

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