Effects of ubiquitin C-terminal hydrolase L1 deficiency on mouse ova

Sayaka Koyanagi, Hiroko Hamasaki, Satoshi Sekiguchi, Kenshiro Hara, Yoshiyuki Ishii, Shigeru Kyuwa, Yasuhiro Yoshikawa

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Maternal proteins are rapidly degraded by the ubiquitin-proteasome system during oocyte maturation in mice. Ubiquitin C-terminal hydrolase L1 (UCHL1) is highly and specifically expressed in mouse ova and is involved in the polyspermy block. However, the role of UCHL1 in the underlying mechanism of polyspermy block is poorly understood. To address this issue, we performed a comprehensive proteomic analysis to identify maternal proteins that were relevant to the role of UCHL1 in mouse ova using UCHL1-deficient gad. Furthermore, we assessed morphological features in gad mouse ova using transmission electron microscopy. NACHT, LRR, and PYD domain-containing (NALP) family proteins and endoplasmic reticulum (ER) chaperones were identified by proteomic analysis. We also found that the 'maternal antigen that embryos require' (NLRP5 (MATER)) protein level increased significantly in gad mouse ova compared with that in wild-type mice. In an ultrastructural study, gad mouse ova contained less ER in the cortex than in wild-type mice. These results provide new insights into the role of UCHL1 in the mechanism of polyspermy block in mouse ova.

Original languageEnglish
Pages (from-to)271-279
Number of pages9
JournalReproduction
Volume143
Issue number3
DOIs
Publication statusPublished - 2012 Mar

ASJC Scopus subject areas

  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Obstetrics and Gynaecology
  • Cell Biology

Fingerprint Dive into the research topics of 'Effects of ubiquitin C-terminal hydrolase L1 deficiency on mouse ova'. Together they form a unique fingerprint.

Cite this