Effects of thyroid hormone on carbonic anhydrase I gene expression in human erythroid cells

Hironobu Hori, Katsumi Yoshida, Hiroshi Fukazawa, Yoshinori Kiso, Noriyo Sayama, Kouki Mori, Yoshihiko Aizawa, Jun Ichi Tani, Sadayoshi Ito

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Abstract

Individuals with hyperthyroidism exhibit concentrations of carbonic anhydrase I (CAI) in red blood cells that reflect the integrated serum thyroid hormone concentration over the preceding few months. Furthermore, triiodothyronine T3, at a physiological free concentration, decreases the CAI concentration in both human erythroleukemic YN-1 cells and burst-forming unit-erythroid (BFU-E)-derived cells. In the present study, the effect of T3 on CAI mRNA levels in various human erythroleukemic cell lines (YN-1, HEL and KU-812) and BFU-E-derived cells was studied. Northern analysis of RNA extracted from erythroid cells revealed a CAI mRNA of 1.5 kilobases. T3 significantly decreased the levels of CAI mRNA in YN-1 and BFU-E-derived cells in a dose-dependent manner. Incubation of T3-stimulated cells with actinomycin D prevented the decrease in CAI mRNA levels. By contrast, T3 had no effect on either the concentrations of CAI or the levels of CAI mRNA in HEL and KU-812 cells. These results suggest that YN-1 and BFU-E-derived cells may be useful models for investigating T3 actions on CAI mRNA in human cells.

Original languageEnglish
Pages (from-to)525-531
Number of pages7
JournalThyroid
Volume8
Issue number6
DOIs
Publication statusPublished - 1998 Jan 1

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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    Hori, H., Yoshida, K., Fukazawa, H., Kiso, Y., Sayama, N., Mori, K., Aizawa, Y., Tani, J. I., & Ito, S. (1998). Effects of thyroid hormone on carbonic anhydrase I gene expression in human erythroid cells. Thyroid, 8(6), 525-531. https://doi.org/10.1089/thy.1998.8.525