Effects of thymic myoid cell culture supernatant on cells from lymphatic tissues

Isao Kamo, Aiko Tada-Kikuchi, Shoei Furukawa, Yuzo Iwasaki, Ikuya Nonaka, Eijiro Satoyoshi, Tsunetoshi Itoh

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Conditioned media (MCM) of cloned thymic myoid cells (IT45R92, R613Ad, and R615B2) were used to investigate their possible involvement in thymic biological events. Those myoid cells produced in a culture medium biological activities capable of stimulating the growth of thymocytes, spleen cells, and bone marrow cells of mice and rats. Surface markers detected on spleen cells proliferating in MCM were characteristic of monocyte-macrophage lineages (C3R, FcγR, asialo GM1) and T-cell lineages (Thy 1) but not B cells (sIgG). Chromatographic studies also suggested that the biological activities of MCM could be separated into two different molecular entities, such as a colony-stimulating activity and an interleukin 1-like activity which supported the growth of monocyte-macrophage lineages and T-cell lineages, respectively. These results indicate that thymic myoid cells produce cytokines important for the regulation of intrathymic interleukin cascade by which clonally differentiated thymic lymphocytes may be expanded into a sizable pool.

Original languageEnglish
Pages (from-to)587-597
Number of pages11
JournalCellular Immunology
Volume94
Issue number2
DOIs
Publication statusPublished - 1985 Sep

ASJC Scopus subject areas

  • Immunology

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