Effects of the Protein Phosphatase Inhibitors Okadaic Acid and Calyculin A on Insulin Release from Rat Pancreatic Islets

Tatsuo Tamagawa, Akihisa Iguchi, Kazumasa Uemura, Hisayuki Miura, Katsunori Nonogaki, Toshiaki Ishiguro, Nobuo Sakamoto

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The role of protein phosphatases in the regulation of insulin release from rat pancreatic islets was studied with protein phosphatase inhibitors, okadaic acid and calyculin A. Okadaic acid inhibited glucose- and glyceraldehyde-induced insulin release dose-dependently and also inhibited the potentiation of glucose-induced release either by adding forskolin, an activator of adenylate cyclase or by increasing K+ concentration to 25 mM. At a non-stimulatory concentration of 3 mM glucose, a high concentration (2 μM) of okadaic acid inhibited insulin release induced by high K+ or 12-O-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C, but a low concentration (1 μM) of okadaic acid did not significantly inhibit TPA-induced insulin release. Calyculin A also inhibited glucose-induced insulin release, and the effect was greater than that of okadaic acid. The data suggest that protein phosphatases may play an important role in the regulation of insulin releae.

Original languageEnglish
Pages (from-to)325-329
Number of pages5
JournalEndocrinologia Japonica
Volume39
Issue number3
DOIs
Publication statusPublished - 1992
Externally publishedYes

Keywords

  • Calyculin A
  • Insulin release
  • Okadaic acid
  • Pancreatic islets

ASJC Scopus subject areas

  • Endocrinology
  • Engineering(all)

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