TY - JOUR
T1 - Effects of the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on bacterial viability and metabolism
AU - Izutani, Naomi
AU - Imazato, Satoshi
AU - Nakajo, Kazuko
AU - Takahashi, Nobuhiro
AU - Takahashi, Yusuke
AU - Ebisu, Shigeyuki
AU - Russell, Roy R.B.
PY - 2011/4
Y1 - 2011/4
N2 - The antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) is a strong bactericide when unpolymerized and has the potential to be utilized in various resinous biomaterials. To analyze the antibacterial characteristics of this monomer in detail, the ability of high concentrations of unpolymerized MDPB to kill Streptococcus mutans in planktonic or biofilm forms within a short time-period of contact, and the inhibitory effects of low concentrations of MDPB on the metabolic function of S. mutans, were examined. High concentrations of MDPB showed effective killing of planktonic and biofilm S. mutans cells within 60s, and complete killing was obtained by contact with 1,000μgml-1 of MDPB for 60s. At a concentration of 4-8μgml-1, MDPB demonstrated growth inhibition, inducing elongation of the lag phase and of the doubling time, when the bacterial number was low. Inhibition of the production of acid from S. mutans by 8μgml-1 of MDPB may have been caused by the inhibition of lactate dehydrogenase activity. At high concentrations, MDPB is lethal to both planktonic and biofilm forms of S. mutans in a short time-period, and at low concentrations, MDPB inhibits metabolic enzymatic activity.
AB - The antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) is a strong bactericide when unpolymerized and has the potential to be utilized in various resinous biomaterials. To analyze the antibacterial characteristics of this monomer in detail, the ability of high concentrations of unpolymerized MDPB to kill Streptococcus mutans in planktonic or biofilm forms within a short time-period of contact, and the inhibitory effects of low concentrations of MDPB on the metabolic function of S. mutans, were examined. High concentrations of MDPB showed effective killing of planktonic and biofilm S. mutans cells within 60s, and complete killing was obtained by contact with 1,000μgml-1 of MDPB for 60s. At a concentration of 4-8μgml-1, MDPB demonstrated growth inhibition, inducing elongation of the lag phase and of the doubling time, when the bacterial number was low. Inhibition of the production of acid from S. mutans by 8μgml-1 of MDPB may have been caused by the inhibition of lactate dehydrogenase activity. At high concentrations, MDPB is lethal to both planktonic and biofilm forms of S. mutans in a short time-period, and at low concentrations, MDPB inhibits metabolic enzymatic activity.
KW - Antibacterial
KW - Biofilm
KW - Dental resins
KW - Monomer
KW - Streptococcus mutans
UR - http://www.scopus.com/inward/record.url?scp=79952655241&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952655241&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0722.2011.00817.x
DO - 10.1111/j.1600-0722.2011.00817.x
M3 - Article
C2 - 21410559
AN - SCOPUS:79952655241
VL - 119
SP - 175
EP - 181
JO - European Journal of Oral Sciences
JF - European Journal of Oral Sciences
SN - 0909-8836
IS - 2
ER -