Effects of sulfate concentration and sludge retention time on the interaction between methane production and sulfate reduction for butyrate

O. Mizuno, Y. Y. Li, T. Noike

Research output: Contribution to journalConference articlepeer-review

70 Citations (Scopus)

Abstract

The effects of sulfate concentration and COD/S ratio on the anaerobic degradation of butyrate were investigated by using 2.0 L anaerobic chemostat-type reactor at 35°C. The study was conducted over a wide range of the COD/S ratio (1.5 to 148) by varying COD concentrations (2500-10000 mg/L) and sulfate concentrations (68-1667 mg-S/L) in the substrate. The sludge retention time at each COD/S ratio was changed from 5 to 20 days. The interaction between methane producing bacteria (MPB) and sulfate-reducing bacteria (SRB) was evidently influenced by COD/S ratio in the substrate. When COD/S ratio was 6.0 or more, methane production was the predominate reaction and over 80% of the total electron flow was used by MPB. At the COD/S ratio of 1.5, SRB utilized over 50% of the total electron flow. A large amount of sulfate reduction resulted in not only the decrease of methane production, but also the rapid increase of the bacterial growth. The degradation pathway of butyrate and the composition of bacterial populations in the reactor were also dominated by COD/S ratio. In sulfate depleted condition, butyrate was degraded to methane via acetate and hydrogen by MPB. On the other hand, butyrate was firstly degraded into sulfide and acetate in sulfate rich conditions by SRB, and the produced acetate was then degraded by acetate consuming MPB and SRB. The methanogenesis from acetate was inhibited by the high concentration of sulfide.

Original languageEnglish
Pages (from-to)45-54
Number of pages10
JournalWater Science and Technology
Volume30
Issue number8 pt 8
DOIs
Publication statusPublished - 1994
EventProceedings of the 17th Biennial Conference of the International Association on Water Quality. Part 8 - Budapest, Hung
Duration: 1994 Jul 241994 Jul 30

Keywords

  • Anaerobic degradation
  • Bacterial population
  • Butyrate
  • Competition
  • Electron flow
  • Metabolic activity
  • Methane-producing bacteria
  • Sulfate-reducing bacteria

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

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