Effects of sphingolipid fractions from golden oyster mushroom (Pleurotus citrinopileatus) on apoptosis induced by inflammatory stress in an intestinal tract in vitro model

Mirinthorn Jutanom, Chisato Higaki, Shinji Yamashita, Kiyotaka Nakagawa, Satoshi Matsumoto, Mikio Kinoshita

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Previously, we reported that the polar lipid fraction from the golden oyster mushroom, Pleurotus citrinopileatus, suppresses colon injuries which result from apoptosis induced by inflammatory stresses in vivo and in vitro (Yamashita et al., J. Oleo Sci., 69, 751-757 (2020)). Here, we investigated the use of lipid classes in mushroom polar lipid fraction in alleviating colon injury using differentiated Caco-2 cells as an intestinal tract model. The mushroom polar lipid fraction was separated into four fractions using silica thin layer chromatography. Each mushroom polar lipid fraction suppressed lipopolysaccharide (LPS)-induced decreases in the viability of intestinal cells, and the effects of sphingolipid fractions were significantly stronger than those of fraction that did not contain sphingolipids. Addition of sphingolipid fractions suppressed the expression of apoptosis-related proteins (e.g., death receptors and caspases) in the LPS-treated cells. Mushroom polar lipids, especially sphingolipids suppress intestinal apoptosis induced by inflammatory stress, and highly polar sphingolipids may exert stronger suppressive effects.

Original languageEnglish
Pages (from-to)1087-1093
Number of pages7
JournalJournal of oleo science
Volume69
Issue number9
DOIs
Publication statusPublished - 2020

Keywords

  • Apoptosis
  • Inflammation
  • Mushroom
  • Sphingoid base
  • Sphingolipid

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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