Effects of selective spinal nerve ligation on acetic acid-induced nociceptive responses and ASIC3 immunoreactivity in the rat dorsal root ganglion

Megumi Omori, Masataka Yokoyama, Yoshikazu Matsuoka, Hiroyuki Kobayashi, Satoshi Mizobuchi, Yoshitaro Itano, Kiyoshi Morita, Hiroyuki Ichikawa

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We investigated changes in pain behavior after injection of acetic acid in the hindpaws of rats with L5 spinal nerve ligation (SNL)-induced neuropathy. We also examined immunoreactivity for acid-sensing ion channel 3 (ASIC3) in the dorsal root ganglion (DRG) of rats with L5 SNL. Two weeks after SNL, the withdrawal threshold to a mechanical stimulus was significantly lower in the SNL group than in the sham-operated group (n = 9 per group, P < 0.01). After acetic acid injection, spontaneous pain responses in the SNL group were significantly increased compared to those in the sham-operated group (n = 5, P < 0.05). L5 SNL significantly increased the proportion of total ASIC3-immunoreactive (ir) neurons in the ipsilateral L4 DRG compared to that in sham-operated rats (n = 4, P < 0.01). Analysis of cell size showed that the proportion of large (> 1200 mm2) ASIC3-ir neurons in the ipsilateral L4 DRG significantly increased after L5 SNL (P < 0.05). In the ipsilateral L5 DRG, the proportion of ASIC3-ir neurons was not significantly affected by treatment. However, L5 SNL significantly increased (P < 0.01) the proportion of small (< 1200 mm2) ASIC3-ir neurons and significantly decreased (P < 0.01) the proportion of large ASIC3-ir neurons compared to proportions in sham-operated animals. These findings suggest that ASIC3 is associated with hyperalgesia in response to a chemical stimulus in the L5 SNL rat model.

Original languageEnglish
Pages (from-to)26-31
Number of pages6
JournalBrain research
Volume1219
DOIs
Publication statusPublished - 2008 Jul 11
Externally publishedYes

Keywords

  • Acetic acid
  • Acid-sensing ion channel 3 (ASIC3)
  • Dorsal root ganglion (DRG)
  • Pain behavior
  • Spinal nerve ligation (SNL)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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