Effects of saturated fatty acids on amylase release from exocrine pancreatic segments of sheep, rats, hamsters, field voles and mice

M. Ohbo, K. Katoh, Y. Sasaki

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Stimulatory effects of saturated fatty acids consisting of 4(butyrate), 8(octanoate), 12(laurate) and 16(palmitate) carbon atoms, as well as acetylcholine on pancreatic amylase release were assessed in tissue segments isolated from sheep, rats, hamsters, field voles and mice. The amount of amylase release induced by the fatty acids (1 μmol · l-1 to 10 mml · l-1) and by acetylcholine (10 nmol · l-1 to 100 μmol · l-1) increased in a concentration-dependent manner, and the maximum response in response to the fatty acids was obtained at the maximal dose used. The maximum increase in amylase release in response to butyrate or octanoate was highly and significantly (r = 0.974, P < 0.001) dependent on the log value of the mean body mass in the following order: sheep > rats > hamsters > field voles > mice. On the other hand, the response to laurate and palmitate was variable among animal species. Addition of atropine (1.4 μmol · l-1) to the medium did not reduce the responses to octanoate stimulation, but significantly reduced acetylcholine-induced responses, implying that the effects of the fatty acids were not mediated through activation of muscarinic acetylcholine receptors. Reduction of calcium ion concentration in the medium significantly inhibited the responses induced by the fatty acids and acetylcholine, suggesting that amylase release depends on extracellular calcium ions.

    Original languageEnglish
    Pages (from-to)305-309
    Number of pages5
    JournalJournal of Comparative Physiology - B Biochemical, Systemic, and Environmental Physiology
    Volume166
    Issue number5
    DOIs
    Publication statusPublished - 1996 Sep

    Keywords

    • Amylase release
    • Exocrine pancreas
    • Fatty acids
    • Rats
    • Sheep

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Biochemistry
    • Physiology
    • Animal Science and Zoology
    • Endocrinology

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